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METHOD OF ANALYTE ORGANISM IDENTIFICATION, INTERNAL STANDARD DNA COMPOSITION FOR USE IN THIS METHOD AND PROCESS FOR PRODUCING THE SAME

外国特許コード F070001769
整理番号 F070001769
掲載日 2007年12月28日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2005JP022188
国際公開番号 WO 2006/059717
国際出願日 平成17年12月2日(2005.12.2)
国際公開日 平成18年6月8日(2006.6.8)
優先権データ
  • 特願2004-350694 (2004.12.3) JP
発明の名称 (英語) METHOD OF ANALYTE ORGANISM IDENTIFICATION, INTERNAL STANDARD DNA COMPOSITION FOR USE IN THIS METHOD AND PROCESS FOR PRODUCING THE SAME
発明の概要(英語) A method of organism identification according to genotype that realizes enhancement of standardization precision, consequently enhancement of identification precision. Further, there is provided means for enhancing of standardization precision. The method may comprise (1) subjecting a double stranded DNA prepared using the genome of analyte organism as a template to a temperature gradient gel electrophoresis or a denaturing gradient gel electrophoresis, (2) extracting a characteristic point of the double stranded DNA from the obtained electrophoretic pattern of the double stranded DNA, and (3) carrying out identification of the analyte organism on the basis of the extracted characteristic point. Further, the method may comprise (4) performing the above gel electrophoresis of double stranded DNA together with two types of internal standard DNAs, (5) extracting a melting start point of each of the internal standard DNAs from the obtained electrophoretic patterns of the two types of internal standard DNAs,and standardizing the above characteristic point on the basis of these melting start points as a reference point, and (6) carrying out identification of the analyte organism on the basis of the standardized characteristic point.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • Japan Science And Technology Agency
  • Saitama University
  • 発明者(英語)
  • Hamano, Keiichi
  • Kozuka, Mariko
  • Mohammed, Naimuddin
  • Nishigaki, Koichi
国際特許分類(IPC)
指定国 AE AG AL AM AT AU AZ BA BB BE BF BG BJ BR BW BY BZ CA CF CG CH CI CM CN CO CR CU CY CZ DE DK DM DZ EC EE EG ES FI FR GA GB GD GE GH GM GN GQ GR GW HR HU ID IE IL IN IS IT JP KE KG KM KN KP KR KZ LC LK LR LS LT LU LV LY MA MC MD MG MK ML MN MR MW MX MZ NA NE NG NI NL NO NZ OM PG PH PL PT RO RU SC SD SE SG SI SK SL SM SN SY SZ TD TG TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW
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