TOP > 外国特許検索 > METHOD OF DETECTING INTERACTION BETWEEN SUBSTANCE AND PROTEIN, METHOD OF SCREENING PROTEIN INTERACTING WITH SUBSTANCE, AND METHOD OF FORMING COMPLEX OF SUBSTANCE AND PROTEIN INTERACTING WITH THE SUBSTANCE

METHOD OF DETECTING INTERACTION BETWEEN SUBSTANCE AND PROTEIN, METHOD OF SCREENING PROTEIN INTERACTING WITH SUBSTANCE, AND METHOD OF FORMING COMPLEX OF SUBSTANCE AND PROTEIN INTERACTING WITH THE SUBSTANCE

外国特許コード F070001807
整理番号 F070001807
掲載日 2008年3月10日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2002JP008078
国際公開番号 WO 2003/014734
国際出願日 平成14年8月7日(2002.8.7)
国際公開日 平成15年2月20日(2003.2.20)
優先権データ
  • 特願2002-172129 (2002.6.12) JP
  • 特願2001-239175 (2001.8.7) JP
発明の名称 (英語) METHOD OF DETECTING INTERACTION BETWEEN SUBSTANCE AND PROTEIN, METHOD OF SCREENING PROTEIN INTERACTING WITH SUBSTANCE, AND METHOD OF FORMING COMPLEX OF SUBSTANCE AND PROTEIN INTERACTING WITH THE SUBSTANCE
発明の概要(英語) In a method of detecting an interaction between a bait and a prey involving the step of bringing the bait into contact with the prey which is a protein and detecting the complex formed by the contact, a fused protein of the pray with a protein usable as a label for detection is formed while the bait is modified for separation. Then mRNA encoding the fused protein containing the pray is translated in a cell-free translation system in the presence of the bait to thereby couple the translation step with the interaction step. Thus, the prey is formed in the cell-free translation system and then brought into contact with the bait. The complex is detected with the use of the modification for separation and the label for detection. Thus, interactions between proteins, nucleic acid-protein, etc. are analyzed in vitroat a high sensitivity. In case where the bait is a protein, alternatively, mRNA encoding the fused protein containing the bait as described above is translated and mRNA encoding the protein containing the prey or the fused protein is also translated in a cell-free cotranslation system to thereby couple the translation and interaction steps. The bait and prey thus formed are allowed to interact with each other and the complex is detected with the use of the modification for separation and the label for detection, thereby analyzing interactions between proteins, nucleic acid-protein, etc. in vitro at a high sensitivity.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • Keio University
  • 発明者(英語)
  • Yanagawa, Hiroshi
  • Miyamoto, Etsuko
  • Matsumura, Nobutaka
  • Doi, Nobuhide
  • Tateyama, Seiji
  • Ishizaka, Masamichi
  • Horisawa, Kenichi
国際特許分類(IPC)
指定国 AE AG AL AM AT AU AZ BA BB BE BF BG BJ BR BY BZ CA CF CG CH CI CM CN CO CR CU CY CZ DE DK DM DZ EC EE ES FI FR GA GB GD GE GH GM GN GQ GR GW HR HU ID IE IL IN IS IT JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MC MD MG MK ML MN MR MW MX MZ NE NL NO NZ OM PH PL PT RO RU SD SE SG SI SK SL SN SZ TD TG TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW
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