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Method for preparation of cartilage cell

外国特許コード F110003033
掲載日 2011年5月13日
出願国 欧州特許庁(EPO)
出願番号 08704109
公報番号 2119767
公報番号 2119767
出願日 平成20年1月23日(2008.1.23)
公報発行日 平成21年11月18日(2009.11.18)
公報発行日 平成28年1月20日(2016.1.20)
国際出願番号 JP2008051327
国際公開番号 WO2008091013
国際出願日 平成20年1月23日(2008.1.23)
国際公開日 平成20年7月31日(2008.7.31)
優先権データ
  • 2008JP051327 (2008.1.23) WO
  • 特願2007-012160 (2007.1.23) JP
発明の名称 (英語) Method for preparation of cartilage cell
発明の概要(英語) The present invention provides a cell derived from a perichondrial tissue, the cell being capable of differentiating into a chondrocyte.
The present invention also provides a method of preparing the above-described cell and a composition comprising the same.
A method of preparing a chondrocyte and a medium for use in the method are also provided.
特許請求の範囲(英語) [claim1]
1. A cell derived from a human perichondrial tissue wherein Alcian blue does not stain said perichondrial tissue, said cell being capable of differentiating into a chondrocyte.
[claim2]
2. A method of preparing the cell according to claim 1, wherein the method comprises culturing cells isolated from a human perichondrial tissue, wherein Alcian blue does not stain said perichondrial tissue.
[claim3]
3. A composition comprising the cell according to claim 1.
[claim4]
4. The composition according to claim 3, which further comprises: (a) a matrix produced by the cell according to claim 1; and/or (b) scaffold.
[claim5]
5. The composition according to claim 3 or 4 which is suitable for use in proliferating a cell derived from a human perichondrial tissue, said cell being capable of differentiating into a chondrocyte.
[claim6]
6. The composition according to claim 3 or 4 for use in: (a) cell transplant; or (b) cell transplant, wherein the cell transplant aims at any one of treatment of congenital auricular deformity, treatment of costicartilage defect, treatment of damage to articular cartilage, treatment of tracheal cartilage defect, rhinoplasty, genioplasty, plastic surgery of small facial recesses, corrective surgery of facial left-right asymmetry, corrective surgery around eyelids, or cosmetic surgery of face.
[claim7]
7. A method of preparing chondrocytes, comprising differentiating the cell according to claim 1 into chondrocytes.
[claim8]
8. The method according to claim 7, wherein (a) cell masses are formed by culturing the cell according to claim 1 in centrifuge tubes; or (b) the cell according to claim 1 is multi-layered by plate culture.
[claim9]
9. The method according to claim 7 or 8, wherein the cell according to claim 1 is proliferated and/or differentiated in a medium containing a serum.
[claim10]
10. The method according to claim 9, wherein the serum is bovine serum or an autoserum.
[claim11]
11. The method according to any one of claims 7 to 10, wherein the cell according to claim 1 is differentiated into chondrocytes in a medium containing DMEM/F12, a serum, antibiotics and antimycotics.
[claim12]
12. The method according to claim 11, wherein the medium further contains dexamethasone and/or L-ascorbic acid.
[claim13]
13. The method according to claim 11 or 12, wherein the medium further contains an insulin-like growth factor and/or a basic fibroblast growth factor.
[claim14]
14. A method of preparing a matrix produced by chondrocytes, comprising differentiating the cell according to claim 1 into chondrocytes and allowing said chondrocytes to produce the matrix.
[claim15]
15. The method according to claim 14, wherein the matrix is type II collagen and/or proteoglycan.
  • 出願人(英語)
  • YOKOHAMA CITY UNIVERSITY
  • 発明者(英語)
  • TANIGUCHI HIDEKI
  • KOBAYASHI SHINJI
国際特許分類(IPC)
指定国 Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR
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