TOP > 外国特許検索 > Method for production of DHA-containing phospholipid through microbial fermentation

Method for production of DHA-containing phospholipid through microbial fermentation 新技術説明会

外国特許コード F110003071
掲載日 2011年5月25日
出願国 アメリカ合衆国
出願番号 45186008
公報番号 20100105113
公報番号 8652814
出願日 平成20年6月3日(2008.6.3)
公報発行日 平成22年4月29日(2010.4.29)
公報発行日 平成26年2月18日(2014.2.18)
国際出願番号 JP2008001394
国際公開番号 WO2008149542
国際出願日 平成20年6月3日(2008.6.3)
国際公開日 平成20年12月11日(2008.12.11)
優先権データ
  • 特願2007-148398 (2007.6.4) JP
  • 2008JP001394 (2008.6.3) WO
発明の名称 (英語) Method for production of DHA-containing phospholipid through microbial fermentation 新技術説明会
発明の概要(英語) Disclosed is a method for producing a DHA phospholipid comprising an omega3 unsaturated fatty acid, particularly DHA, as a constituent lipid by using a microorganism in a simpler manner.
Specifically disclosed is a method for producing a phospholipid comprising an omega3 unsaturated fatty acid as a constituent lipid, which comprises the steps of: growing a microorganism capable of producing the omega3 unsaturated fatty acid in a culture medium containing a carbon source; and further culturing the grown microorganism in a culture medium without any carbon source.
The method enables to produce a highly value-added phospholipid which comprises an omega3 unsaturated fatty acid as a constituent lipid by using a microorganism capable of producing the omega3 unsaturated fatty acid in a large quantity.
特許請求の範囲(英語) [claim1]
1. A method for increasing the content of a phospholipid comprising an omega 3 unsaturated fatty acid as a constituent lipid in a microorganism capable of producing the omega 3 unsaturated fatty acid, comprising the steps of: (a) growing the microorganism in a culture medium containing a carbon source, whereby the phospholipid is produced at a first level and stored in a microbial cell body of the microorganism; and
(b) further growing the microorganism from step (a) in a culture medium without any carbon source, whereby the content of the phospholipid in the microorganism is increased to a second level that is greater than the first level.
[claim2]
2. The method as set forth in claim 1, wherein the microorganism is a labyrinthulean microorganism or thraustochytride microorganism.
[claim3]
3. The method as set forth in claim 2, wherein the labyrinthulean microorganism is labyrinthulean strain 12B.
[claim4]
4. The method as set forth in claim 2, wherein the labyrinthulean microorganism is selected from the group consisting of genus Labyrinthula microorganisms, genus Thraustochytrium microorganisms and genus Schizochytrium microorganisms.
[claim5]
5. The method as set forth in claim 1, wherein the omega 3 unsaturated fatty acid is docosahexaenoic acid.
[claim6]
6. The method as set forth in claim 2, wherein the omega 3 unsaturated fatty acid is docosahexaenoic acid.
[claim7]
7. The method as set forth in claim 3, wherein the omega 3 unsaturated fatty acid is docosahexaenoic acid.
[claim8]
8. The method as set forth in claim 4, wherein the omega 3 unsaturated fatty acid is docosahexaenoic acid.
[claim9]
9. The method as set forth in claim 1, wherein step (b) is carried out under forced aeration.
[claim10]
10. The method as set forth in claim 2, wherein step (b) is carried out under forced aeration.
[claim11]
11. The method as set forth in claim 3, wherein step (b) is carried out under forced aeration.
[claim12]
12. The method as set forth in claim 4, wherein step (b) is carried out under forced aeration.
[claim13]
13. The method as set forth in claim 5, wherein step (b) is carried out under forced aeration.
[claim14]
14. A method for increasing the content of a phospholipid comprising a docosahexaenoic acid as a constituent lipid in labyrinthulean strain 12B, comprising the steps of: (a) growing the labyrinthulean strain 12B in a culture medium containing a carbon source, whereby the phospholipid is produced at a first level and stored in a microbial cell body of the microorganism; and
(b) further growing the labyrinthulean strain 12B from step (a) in a culture medium without any carbon source, whereby the content of the phospholipid in the microorganism is increased to a second level that is greater than the first level.
[claim15]
15. The method as set forth in claim 14, wherein the microorganism is grown under forced aeration.
  • 発明者/出願人(英語)
  • OKUYAMA HIDETOSHI
  • ORIKASA YOSHITAKE
  • NISHIDA TAKANORI
  • HOKKAIDO UNIVERSITY
  • RESEARCH OF MICROBES
国際特許分類(IPC)
米国特許分類/主・副
  • 435/134
ライセンスをご希望の方、特許の内容に興味を持たれた方は、下記までご連絡ください

PAGE TOP

close
close
close
close
close
close