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Reagent for introduction of protein or gene

外国特許コード F110003080
整理番号 134-EP
掲載日 2011年6月6日
出願国 欧州特許庁(EPO)
出願番号 07849971
公報番号 2116599
公報番号 2116599
出願日 平成19年11月26日(2007.11.26)
公報発行日 平成21年11月11日(2009.11.11)
公報発行日 平成26年8月6日(2014.8.6)
国際出願番号 JP2007073266
国際公開番号 WO2008062911
国際出願日 平成19年11月26日(2007.11.26)
国際公開日 平成20年5月29日(2008.5.29)
優先権データ
  • 2007JP073266 (2007.11.26) WO
  • 特願2006-317841 (2006.11.24) JP
発明の名称 (英語) Reagent for introduction of protein or gene
発明の概要(英語) The present invention provides a reagent for introducing a protein or gene into a cell.
The reagent of the present invention is, for example, a reagent for introducing a protein or gene into a cell, which comprises a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1: (see diagramm) (wherein in formula (I)-1: L is a single bond, -CONH-, or -S-S-; M **1 is -(CH 2) k- or -(CH 2CH 2O) k- (wherein k is an integer between 0 and 14); and m1 and m2 are each independently an integer between 11 and 21 (in this regard, when providing a reagent for introducing a gene into a cell, the case where both m1 and m2 are 15 is excluded)).
特許請求の範囲(英語) [claim1]
1. Use of a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1, (II)-1 or (III)-1: (see diagramm) wherein: L is -CONH-, or -S-S-; M **1 is -(CH 2) k- and k is an integer between 0 and 7; and m1 and m2 are each independently an integer between 11 and 21; (see diagramm) wherein: L is -CONH-, or -S-S-; M **1 is -(CH 2) k- and k is an integer between 0 and 7; and m1 and m2 are each independently an integer between 11 and 21; or (see diagramm) wherein: L is -CONH-, or -S-S-; M **1 is -(CH 2) k- and k is an integer between 0 and 7; and m1 and m2 are each independently an integer between 11 and 21,
for introducing a protein into a cell.
[claim2]
2. Use of a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1, (II)-1 or (III)-1: (see diagramm) wherein: L is -CONH-, or -S-S-; M **1 is -(CH 2) k- and k is an integer between 0 and 7; and m1 and m2 are each independently an integer between 11 and 21 with the proviso that the case where both m1 and m2 are 15 is excluded; (see diagramm) wherein: L is -CONH-, or -S-S-; M **1 is -(CH 2) k- and k is an integer between 0 and 7; and m1 and m2 are each independently an integer between 11 and 21; or (see diagramm) wherein: L is -CONH-, or -S-S-; M **1 is -(CH 2) k- and k is an integer between 0 and 7; and m1 and m2 are each independently an integer between 11 and 21,
for introducing a gene into a cell.
[claim3]
3. The use according to claim 1 or 2, wherein the composition further comprises at least one substance selected from the group consisting of diacylphosphatidylcholine, cholesterol, and an amphipathic molecule to which a polyethylene glycol chain is bound.
[claim4]
4. The use according to claim 3, wherein one or two acyl chains of diacylphosphatidylcholine is an oleoyl group.
[claim5]
5. The use according to claim 1 or 2, wherein the composition is a molecular assembly dispersed in an aqueous medium.
[claim6]
6. The use according to claim 5, wherein the molecular assembly forms a bilayer membrane vesicle structure, and wherein an inner aqueous phase thereof includes a protein or gene.
[claim7]
7. The use according to claim 1 or 2, wherein the composition is a dry powder product.
[claim8]
8. The use according to claim 7, wherein the dry powder product can form a bilayer membrane vesicle structure including a protein or gene when dispersed in an aqueous medium.
[claim9]
9. The use according to claim 1 or 2, wherein the cell is: a cell constituting a part of body tissue; plasma, serum or blood; or a cell cultured in a medium containing a part of components thereof.
[claim10]
10. A kit for introducing a protein or gene into a cell, which comprises the composition as defined in any one of claims 1 to 9.
[claim11]
11. A cationic amino acid type lipid represented by the following formula (I)-3, (II)-3 or (III)-3: (see diagramm) wherein: M **2 is -(CH 2) k'- and k' is an integer between 0 and 7; and p1 and p2 are each independently an integer between 11 and 21; (see diagramm) wherein: M **2 is -(CH 2) k'- and k' is an integer between 0 and 7; and p1 and p2 are each independently an integer between 11 and 21; or (see diagramm) wherein: M **2 is -(CH 2) k'- and k' is an integer between 0 and 7; and p1 and p2 are each independently an integer between 11 and 21.
  • 出願人(英語)
  • WASEDA UNIVERSITY
  • 発明者(英語)
  • TAKEOKA SHINJI
  • TAKEDA NAOYA
  • KURUMIZAKA HITOSHI
  • SAKANE ISAO
  • IKEGAYA NAMIKO
  • OBATA YOSUKE
  • SAITO SYUNSUKE
国際特許分類(IPC)
欧州特許分類/主・副
  • C07C237/08
  • C07C237/12
  • C07C237/22
  • C07K005/02D
  • C07K005/06B
  • C07K005/08B
指定国 Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR
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