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Method of inducing homologous recombination of somatic cell

外国特許コード F110003491
整理番号 A132-14EP
掲載日 2011年6月28日
出願国 欧州特許庁(EPO)
出願番号 03781013
公報番号 1584678
公報番号 1584678
出願日 平成15年12月22日(2003.12.22)
公報発行日 平成17年10月12日(2005.10.12)
公報発行日 平成28年8月31日(2016.8.31)
国際出願番号 JP2003016496
国際公開番号 WO2004058964
国際出願日 平成15年12月22日(2003.12.22)
国際公開日 平成16年7月15日(2004.7.15)
優先権データ
  • 2003JP016496 (2003.12.22) WO
  • 特願2002-376555 (2002.12.26) JP
発明の名称 (英語) Method of inducing homologous recombination of somatic cell
発明の概要(英語) The present invention provides a method for obtaining diverse novel genes by inducing somatic cell homologous recombination at genetic loci in somatic cells. <??>By controlling transcription activity of a gene that exists at a genetic locus in a eukaryotic organism cell wherein DNA homologous recombination is occurring at an arbitrary genetic locus, somatic cell homologous recombination is induced between said gene and a gene having a DNA sequence similar to said gene that exists in a region upstream of a transcription promoter, whereby it is possible to obtain diverse novel genes having a plurality of genetic information. <IMAGE>
特許請求の範囲(英語) [claim1]
1. A method for inducing homologous recombination between a gene and a base sequence having 60%, notably 70%, in particular, 80% and, more particularly, 90% or greater sequence identity to the entirety of the gene for which homologous recombination is induced in a DT40 cell, comprising controlling transcription of said gene by a transcriptional promoter in the DT40 cell,
wherein said transcriptional promoter is operably linked to said gene,
wherein said base sequence is located on the upstream 5' side of said gene,
wherein said base sequence is located on the upstream 5' side of said transcriptional promoter, and
wherein said gene is an exogenous gene.
[claim2]
2. A method recited in Claim 1, characterized in that a cis-acting region for a transcription control contains either one or both of an enhancer and a nuclear matrix attachment region (MAR).
[claim3]
3. A method recited in Claim 1 or 2, wherein said gene and said base sequence are placed on a vector and wherein said transcriptional promoter is located between said base sequence and said gene.
[claim4]
4. A method recited in Claim 3, characterized in that either one or both of an enhancer and a nuclear matrix attachment region (MAR) are inserted onto the aforementioned vector and are operably linked to the aforementioned transcription promoter.
[claim5]
5. A method recited in Claim 3 or 4, characterized in that the aforementioned transcription promoter is an inducible promoter.
[claim6]
6. A method recited in Claim 5, characterized in that the aforementioned inducible promoter is a tetracycline inducible promoter.
[claim7]
7. A method recited in any one of Claims 3 through 6, characterized in that the aforementioned gene is an enhanced cyan fluorescent protein (ECFP) gene.
[claim8]
8. A method recited in any one of Claims 3 through 7, characterized in that the base sequence is an enhanced green fluorescent protein (EGFP) genetic sequence.
[claim9]
9. A method recited in any one of Claims 2 through 8, characterized in that the aforementioned enhancer is a chicken antibody light chain gene enhancer (3' enhancer), and the aforementioned nuclear matrix attachment region (MAR) is chicken derived.
  • 出願人(英語)
  • RIKEN
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • CHIOME BIOSCIENCE
  • 発明者(英語)
  • OHTA KUNIHIRO
  • SEO HIDETAKA
  • SHIBATA TAKEHIKO
国際特許分類(IPC)
欧州特許分類/主・副
  • C12N015/90B
指定国 Contracting States: CH DE FR GB LI
参考情報 (研究プロジェクト等) CREST Structure and Function of Genomes AREA
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