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Viral vector and use thereof

外国特許コード F110003610
整理番号 A181-23WO
掲載日 2011年6月30日
出願国 欧州特許庁(EPO)
出願番号 08740267
公報番号 2154246
公報番号 2154246
出願日 平成20年4月11日(2008.4.11)
公報発行日 平成22年2月17日(2010.2.17)
公報発行日 平成29年3月29日(2017.3.29)
国際出願番号 JP2008057170
国際公開番号 WO2008136253
国際出願日 平成20年4月11日(2008.4.11)
国際公開日 平成20年11月13日(2008.11.13)
優先権データ
  • 特願2007-120181 (2007.4.27) JP
  • 2008WO-JP57170 (2008.4.11) WO
発明の名称 (英語) Viral vector and use thereof
発明の概要(英語) (EP2154246)
It is intended to provide a polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein, and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, the linking site being located downstream of the second base sequence, the second base sequence being obtained by modifying with a base sequence in a native sequence derived from a virus by insertion, substitution, or addition.
By using this, a vector containing a viral base sequence is constructed, and a protein is efficiently produced without worsening growth of a host cell containing the vector.
特許請求の範囲(英語) [claim1]
1. A polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein; and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, and the linking site being located downstream of the second base sequence, the second base sequence comprising an inserted base sequence having a base length of 100 or more at any position from 17th base to 795th base of the base sequence shown in SEQ ID NO: 20, wherein a polynucleotide having the second base sequence is: (i) a polynucleotide having the base sequence shown in any one of SEQ ID NO: 4 through 17.
[claim2]
2. The polynucleotide according to any one of claims 1, wherein
the viral replication protein is: (i) polypeptides having amino acid sequences shown in SEQ ID NO: 1 and 2, respectively.
[claim3]
3. The polynucleotide according to any of claims 1 or 2, wherein
the virus belongs to a tobamovirus.
[claim4]
4. The polynucleotide according to any of claims 1 to 3, wherein
the virus is a tobacco mosaic virus or a tomato mosaic virus.
[claim5]
5. A vector comprising a polynucleotide recited in any of claims 1 to 4.
[claim6]
6. A plant comprising a polynucleotide recited in any of claims 1 to 4.
[claim7]
7. A plant comprising a vector recited in claim 5.
[claim8]
8. A transformant comprising a polynucleotide recited in claims 1 to 4.
[claim9]
9. A transformant comprising a vector recited in claim 5.
[claim10]
10. A method for producing a polypeptide, comprising: transforming or transfecting a plant with a polynucleotide recited in any of claims 1 to 4.
[claim11]
11. A method for producing a polypeptide, comprising the step of: transforming a cell with a polynucleotide recited in any of claims 1 to 4.
[claim12]
12. A kit for producing a polypeptide, comprising a polynucleotide recited in any of claims 1 to 4.
[claim13]
13. A method for producing a polypeptide, comprising the step of: transforming or transfecting a plant with a vector recited in claim 5.
[claim14]
14. A method for producing a polypeptide, comprising the step of: transforming a cell with a vector recited in claim 5.
[claim15]
15. A kit for producing a polypeptide, comprising a vector recited in claim 5.
[claim16]
16. A method for producing a polypeptide, comprising the step of: using a plant recited in claim 6 or claim 7.
[claim17]
17. A method for producing a polypeptide, comprising the step of: using a transformant recited in claim 8 or claim 9.
[claim18]
18. A kit for producing a polypeptide, comprising a plant recited in claim 6 or 7.
[claim19]
19. A kit for producing a polypeptide, comprising a transformant recited in claim 8 or 9.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • MORI MASASHI
  • DOHI KOJI
国際特許分類(IPC)
欧州特許分類/主・副
  • C12N015/82A4A
  • C12N015/82C4D
  • M12N770/00043
指定国 (EP2154246)
Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR
参考情報 (研究プロジェクト等) CREST Plants Function and Their Control AREA
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