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Use of ip3 receptor-binding protein for controlling intracellular pH

外国特許コード F110003963
整理番号 I018P009EP2
掲載日 2011年7月7日
出願国 欧州特許庁(EPO)
出願番号 10191554
公報番号 2311478
公報番号 2311478
出願日 平成19年3月20日(2007.3.20)
公報発行日 平成23年4月20日(2011.4.20)
公報発行日 平成26年7月16日(2014.7.16)
優先権データ
  • 07739967 (2007.3.20) EP
  • 特願2006-077607 (2006.3.20) JP
発明の名称 (英語) Use of ip3 receptor-binding protein for controlling intracellular pH
発明の概要(英語) The present invention provides a composition comprising an IP 3 receptor-binding protein (IRBIT), a nucleic acid that controls the expression and translation of IRBIT, or an antibody against IRBIT for controlling at least one intracellular biological function selected from the group consisting of (1) protein synthesis, (2) phosphatidylinositol metabolism, and (3) intracellular pH.
特許請求の範囲(英語) [claim1]
1. Use of inositol 1,4,5-triphosphate (IP 3) receptor-binding protein (IRBIT) for controlling intracellular pH in a cell in vitro in which p-type Na/HCO 3 cotransporter 1 (pNBC1) is involved, wherein the IRBIT is a protein comprising an amino acid sequence shown in SEQ ID NO: 1 or SEQ ID NO: 3 or a protein comprising an amino acid sequence having 90% or more identity with the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 3 and having the same biological activity as IRBIT.
[claim2]
2. Use of a DNA encoding IRBIT or vector comprising the DNA for controlling intracellular pH in a cell in vitro in which p-type Na/HCO 3 cotransporter 1 (pNBC1) is involved, wherein the IRBIT is a protein comprising an amino acid sequence shown in SEQ ID NO: 1 or SEQ ID NO: 3 or a protein comprising an amino acid sequence having 90% or more identity with the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 3 and having the same biological activity as IRBIT, and wherein the nucleic acid is selected from the group consisting of DNAs encoding IRBIT.
[claim3]
3. Use of a siRNA for IRBIT for controlling intracellular pH in a cell in vitro in which p-type Na/HCO 3 cotransporter I (pNBC1) is involved, wherein the IRBIT is a protein comprising an amino acid sequence shown in SEQ ID NO: 1 or SEQ ID NO: 3 or a protein comprising an amino acid sequence having 90% or more identity with the amino acid sequence of SEQ ID NO: I or SEQ ID NO: 3 and having the same biological activity as IRBIT, and wherein the siRNA consists of the nucleotide sequence of SEQ ID NO: 5 or 6.
[claim4]
4. Use of an antibody against IRBIT or a fragment thereof, capable of binding specifically to IRBIT, for controlling intracellular pH in a cell in vitro in which p-type Na/HCO 3 cotransporter 1 (pNBC1) is involved, wherein the IRBIT is a protein comprising an amino acid sequence shown in SEQ ID NO: 1 or SEQ ID NO: 3 or a protein comprising an amino acid sequence having 90% or more identity with the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 3 and having the same biological activity as IRBIT, and wherein the fragment is selected from the group consisting of Fab, F(ab') 2, Fv and scfv.
[claim5]
5. The use according to any one of claims 1-4, wherein the control is suppression or elevation.
[claim6]
6. The use according to claim 4, wherein the antibody is a monoclonal antibody, a polyclonal antibody, a recombinant antibody, or a single chain antibody.
[claim7]
7. The use according to any one of claims 1 and 4, wherein the IRBIT is phosphorylated.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • MIKOSHIBA KATSUHIKO
  • ANDO HIDEAKI
  • MIZUTANI AKIHIRO
国際特許分類(IPC)
欧州特許分類/主・副
  • C07K014/47
  • K61K038/00
指定国 Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR
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