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Protein-responsive translation control system utilizing rna-protein interaction motif

外国特許コード F110003968
整理番号 I029P004WO
掲載日 2011年7月7日
出願国 欧州特許庁(EPO)
出願番号 08851746
公報番号 2221367
公報番号 2221367
出願日 平成20年11月21日(2008.11.21)
公報発行日 平成22年8月25日(2010.8.25)
公報発行日 平成29年7月12日(2017.7.12)
国際出願番号 JP2008071213
国際公開番号 WO2009066757
国際出願日 平成20年11月21日(2008.11.21)
国際公開日 平成21年5月28日(2009.5.28)
優先権データ
  • 特願2007-303662 (2007.11.22) JP
  • 特願2008-186385 (2008.7.17) JP
  • 2008WO-JP71213 (2008.11.21) WO
発明の名称 (英語) Protein-responsive translation control system utilizing rna-protein interaction motif
発明の概要(英語) (EP2221367)
An object of the present invention is to provide a translationally regulatable mRNA which has wider application and can perform specific ON-OFF regulation, an RNA-protein complex specifically bound to the mRNA, and a translational regulatory system.
The present invention provides an mRNA having an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5' region of the open reading frame, and an mRNA having a nucleotide sequence complementary to an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5' region of the open reading frame.
特許請求の範囲(英語) [claim1]
1. An mRNA having an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5' region of an open reading frame.
[claim2]
2. An mRNA having a nucleotide sequence complementary to an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5' region of an open reading frame.
[claim3]
3. The mRNA according to claim 1 or 2, wherein the interacting motif is an L7Ae-derived nucleotide sequence.
[claim4]
4. The mRNA according to claim 1 or 2, wherein the interacting motif is a threonyl-tRNA synthetase (ThrRS)-derived nucleotide sequence.
[claim5]
5. An RNA-protein complex comprising an mRNA according to claim 1 and a protein specifically binding to the nucleotide sequence.
[claim6]
6. A translational regulatory system comprising an mRNA according to claim 1 and a protein specifically binding to the nucleotide sequence.
[claim7]
7. A method for translational regulation of mRNA, comprising contacting an mRNA according to claim 1 with a protein specifically binding to the protein-binding motif.
[claim8]
8. A translational regulatory system comprising (a) an mRNA having a nucleotide sequence complementary to an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5' region of the open reading frame, (b) an RNA having the RNA-protein complex interacting motif-derived nucleotide sequence, and (c) a protein specifically binding to the RNA (b).
[claim9]
9. An artificial information conversion system which converts input information of an arbitrary substrate protein to output information of an arbitrary target protein.
[claim10]
10. A simultaneous translational regulatory system which regulates the translational repression and activation of different genes using one protein, the system comprising (a) an mRNA having a nucleotide sequence complementary to an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5' region of the open reading frame, (b) an RNA having the RNA-protein complex interacting motif-derived nucleotide sequence, (c) a protein specifically binding to the RNA (b), and (d) an mRNA having a nucleotide sequence identical to the nucleotide sequence in the RNA (b), 5' to the ribosome-binding site or within the 5' region of the open reading frame, the mRNA encoding a gene different from that encoded by the mRNA (a).
[claim11]
11. A plasmid vector comprising a nucleic acid sequence encoding an mRNA according to claim 1 or 2.
[claim12]
12. An intracellular translational regulatory system comprising
a first plasmid vector comprising a nucleic acid sequence encoding an mRNA according to claim 1, and
a second plasmid vector comprising a nucleic acid sequence encoding a protein specifically binding to the RNA-protein complex interacting motif-derived nucleotide sequence in the mRNA produced by the first vector.
[claim13]
13. The system according to claim 12, for regulating protein translation in a human cancer cell.
[claim14]
14. A translational regulatory system comprising a fusion protein containing L7Ae as a tag sequence and a first protein.
[claim15]
15. The translational regulatory system according to claim 14, further comprising an mRNA having a sequence specifically binding to L7Ae and a sequence encoding a second protein.
[claim16]
16. An intracellular translational regulatory system comprising a plasmid vector containing a nucleic acid sequence encoding an mRNA encoding L7Ae and a first protein.
[claim17]
17. The intracellular translational regulatory system according to claim 16, further comprising a plasmid vector containing a nucleic acid sequence encoding an mRNA having a sequence specifically binding to L7Ae, the mRNA encoding a second protein.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • INOUE TAN
  • SAITO HIROHIDE
  • KOBAYASHI TETSUHIRO
  • HARA TOMOAKI
国際特許分類(IPC)
欧州特許分類/主・副
  • C12N015/67
指定国 (EP2221367)
Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR
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