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Method and apparatus for collecting micromaterial

外国特許コード F110003994
整理番号 K01501EP
掲載日 2011年7月7日
出願国 欧州特許庁(EPO)
出願番号 03701085
公報番号 1504812
公報番号 1504812
出願日 平成15年1月14日(2003.1.14)
公報発行日 平成17年2月9日(2005.2.9)
公報発行日 平成26年1月1日(2014.1.1)
国際出願番号 JP2003000231
国際公開番号 WO2003095088
国際出願日 平成15年1月14日(2003.1.14)
国際公開日 平成15年11月20日(2003.11.20)
優先権データ
  • 2003JP000231 (2003.1.14) WO
  • 特願2002-131656 (2002.5.7) JP
発明の名称 (英語) Method and apparatus for collecting micromaterial
発明の概要(英語) A recovery method for specific micromaterials such as DNA molecules, cells, microorganisms and the like, approximately several μm in size, from a mixture containing other micromaterials is presented.
A substance (for example, methylcellulose) that undergoes a reversible gel to sol phase transition is utilized, and a micromaterial is accurately and conveniently recovered.
The present invention is a method of recovering a desired micromaterial in a system comprising a medium that undergoes a reversible phase transition between a sol and a gel, a support material and micromaterials, comprising the steps of converting the medium surrounding the micromaterial to be recovered into a gel locally to immobilize said micromaterial on said support along with the gelled medium, removing the medium and micromaterials not immobilized on said support material and converting the gelled medium to a sol to recover the micromaterial.
特許請求の範囲(英語) [claim1]
1. A method of recovering a desired micromaterial in a system comprising a medium that undergoes a reversible phase transition between a sol and a gel, a support material and micromaterials, comprising the steps of converting the medium surrounding the micromaterial to be recovered into a gel locally to immobilize said micromaterial on said support along with the gelled medium, removing the medium and micromaterials not immobilized on said support material and converting the gelled medium to a sol to recover the micromaterial.
[claim2]
2. The method as in claim 1 wherein the medium has a sol to gel phase transition temperature, forms a gel when heated to at or above said phase transition temperature and forms a sol when cooled to at or below said phase transition temperature.
[claim3]
3. The method as in claim 2 wherein the phase transition temperature is from 20 deg.C to 90 deg.C.
[claim4]
4. The method as in claim 2 or 3 wherein the medium is a water soluble cellulose derivative or a solution thereof.
[claim5]
5. The method as in claim 4 wherein the cellulose derivative is methylcellulose.
[claim6]
6. The method as in any preceding claim wherein the support material is transparent.
[claim7]
7. The method as in any preceding claim wherein the support material is an ITO transparent electrode.
[claim8]
8. The method as in any preceding claim wherein the micromaterial has a marker for identification.
[claim9]
9. The method as in any preceding claim wherein the micromaterial is a DNA molecule, a cell or a microorganism.
[claim10]
10. The method as in any one of claims 2 to 9 wherein the medium is heated to a temperature at or above the phase transition temperature by applying infrared irradiation and the medium is cooled to a temperature at or below the phase transition temperature by terminating the infrared irradiation.
[claim11]
11. The method as in claim 10 wherein the means to heat is an infrared laser.
[claim12]
12. The method as in claim 11 wherein the infrared laser is a YAG laser or an Nd:YVO 4 laser.
[claim13]
13. A device for recovering micromaterials comprising a space to retain a medium that undergoes a reversible phase transition between a sol and a gel by changing temperature and a support material, means to heat said support material to a temperature of at least the phase transition temperature of said medium, means to heat said support material locally to a temperature of at least the phase transition temperature of said medium, a flow channel through which said medium can be added to said space, a flow channel through which a cleaning fluid can be added to said space, a flow channel through which the cleaning fluid can be discharged from said space and a flow channel through which said medium can be discharged from said space, wherein the support material is transparent.
[claim14]
14. The device as in claim 13 wherein the medium is a water soluble cellulose derivative or a solution thereof.
[claim15]
15. The device as in claim 14 wherein the cellulose derivative is methylcellulose.
[claim16]
16. The device as in any one of claim 13 to 15 wherein the support material is an ITO transparent electrode.
[claim17]
17. The device as in any one of claims 13 to 16 the means to heat said support material locally being an infrared irradiation device to irradiate the space.
[claim18]
18. The device as in claim 17 wherein the infrared irradiation device is an infrared laser.
[claim19]
19. The device as in claim 18 wherein the infrared laser is a YAG laser or an Nd:YVO 4 laser.
[claim20]
20. The device as in claim 19 further equipped with a lens that concentrates the infrared radiation from an infrared irradiation device to the space.
[claim21]
21. The device as in any one of claims 13 to 20 further equipped with a microscope enabling observation of the space.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • ARAI FUMIHITO
  • FUKUDA TOSHIO
  • ICHIKAWA AKIHIKO
  • KATSURAGI TOHORU
国際特許分類(IPC)
欧州特許分類/主・副
  • L01J219/00C2K
  • L01J219/00C2L8
  • L01J219/00C4L3D
  • L01L200/06J8
  • L01L300/08B4
  • L01L300/18A8
  • L01L300/18E
  • L01L300/18E4
  • L01L400/06P
  • M40B060/14
  • S01N001/28F1
  • S01N001/28F3L
  • S01N001/28M2L
  • S01N015/14G3
  • S01N015/14Y
指定国 Contracting States: CH DE FR GB LI NL SE
参考情報 (研究プロジェクト等) PRESTO Interaction and Intelligence AREA
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