TOP > 外国特許検索 > Photoresponsive nucleic acid manufacturing method

Photoresponsive nucleic acid manufacturing method

外国特許コード F110004125
整理番号 RX02P34WO
掲載日 2011年7月11日
出願国 欧州特許庁(EPO)
出願番号 09710413
公報番号 2253640
公報番号 2253640
出願日 平成21年2月13日(2009.2.13)
公報発行日 平成22年11月24日(2010.11.24)
公報発行日 平成26年7月30日(2014.7.30)
国際出願番号 JP2009000562
国際公開番号 WO2009101810
国際出願日 平成21年2月13日(2009.2.13)
国際公開日 平成21年8月20日(2009.8.20)
優先権データ
  • 2009JP000562 (2009.2.13) WO
  • 特願2008-033713 (2008.2.14) JP
発明の名称 (英語) Photoresponsive nucleic acid manufacturing method
発明の概要(英語) The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology.
The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent.
特許請求の範囲(英語) [claim1]
1. A method of manufacturing a nucleic acid having a group represented by the following Formula XI: (see diagramm)(in the Formula XI, X represents O, S or NH, R1 represents a hydrogen atom, a cyano group, a carboxamide group, an alkoxycarbonyl group, or a monovalent group of a substituted or unsubstituted aromatic compound, and R2 represents a hydrogen atom, a C1 to C6 alkyl group, a C1 to C6 alkoxy group, a cyano group or a C1 to C6 acyl group.), wherein the method comprises the following step (a): (a) a nucleic acid having the Formula I as a base moiety: (see diagramm)(in the Formula I, X represents O, S or NH, and A1 represents a halogen atom.) is reacted with the compound that is represented by the following Formula II (see diagramm)(in the Formula II, R1 represents a hydrogen atom, a cyano group, a carboxamide group, an alkoxycarbonyl group, or a monovalent group of a substituted or unsubstituted aromatic compound, and R2 represents a hydrogen atom, a C1 to C6 alkyl group, a C1 to C6 alkoxy group, a cyano group or a C1 to C6 acyl group.) in the presence of a palladium complex catalyst, a basic substance and a solvent by microwave heating; or a method of manufacturing a nucleic acid having a group represented by the following Formula XV: (see diagramm)(in the Formula XV, X represents O, S or NH, R4 represents a hydrogen atom, a C1 to C6 alkyl group, a C1 to C6 alkoxy group, a cyano group or a C1 to C6 acyl group, and R3 represents a monovalent group of a substituted or unsubstituted aromatic compound, a hydrogen atom, a cyano group, a carboxamide group or an alkoxycarbonyl group.), wherein the method comprises the following step (b):(b) a nucleic acid having the Formula VI as a base moiety: (see diagramm)(in the Formula VI, X represents O, S or NH, R4 represents a hydrogen atom, a C1 to C6 alkyl group, a C1 to C6 alkoxy group, a cyano group or a C1 to C6 acyl group, and A2 represents a halogen atom.) is reacted with the compound that is represented by the following Formula VII:

R 3-B(OH) 2 (VII)

(in the Formula VII, R3 represents a monovalent group of a substituted or unsubstituted aromatic compound, a hydrogen atom, a cyano group, a carboxamide group or an alkoxycarbonyl group.) in the presence of a palladium complex catalyst, a basic substance and a solvent by microwave heating.
[claim2]
2. The method according to Claim 1, comprising the step (a).
[claim3]
3. The method according to Claim 1, comprising the step (b).
[claim4]
4. The method according to Claim 1 or 2, wherein the step (a) is carried out by heating by microwaves in the presence of a palladium complex catalyst, a basic substance, a solvent, and an aqueous solution of carboxylate.
[claim5]
5. The method according to Claim 1 or 3, wherein the step (b) is carried out by heating by microwaves in the presence of a palladium complex catalyst, a basic substance, a solvent, and an aqueous solution of carboxylate.
[claim6]
6. The method according to any one of Claims 1 to 5, wherein the basic substance is trialkylamine having a C1 to C6 alkyl group.
[claim7]
7. The method according to Claim 1 or 2, wherein the step (a) is carried out by heating by microwaves in the presence of a palladium complex catalyst, a solvent, and an aqueous solution of carboxylate.
[claim8]
8. The method according to Claim 1 or 3, wherein the step (b) is carried out by heating by microwaves in the presence of a palladium complex catalyst, a solvent, and an aqueous solution of carboxylate.
[claim9]
9. The method according to any one of Claims 4 to 8, wherein the aqueous solution of carboxylate is an aqueous solution of an alkali metal salt of C1 to C3 carboxylic acid.
[claim10]
10. The method according to any one of Claims 4 to 9, wherein the aqueous solution of carboxylate is a buffer solution having a pH range of 4.5 to 6.0.
[claim11]
11. The method according to any one of Claims 1 to 10, wherein the heating by microwaves is carried out in the temperature range of 70 to 140 deg.C.
[claim12]
12. The method according to any one of Claims 1 to 11, wherein the heating by microwaves is carried out in the time range of 1 to 30 minutes.
[claim13]
13. The method according to any one of Claims 1 to 12, wherein the solvent is an aprotic polar solvent.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • FUJIMOTO KENZO
  • OGINO MASAYUKI
  • YOSHIMURA YOSHINAGA
国際特許分類(IPC)
指定国 Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR
ライセンスをご希望の方、特許の内容に興味を持たれた方は、問合せボタンを押してください。

PAGE TOP

close
close
close
close
close
close