TOP > 外国特許検索 > PROBES FOR DETECTING PROTEIN NUCLEAR TRANSPORT AND METHOD FOR DETECTING AND QUANTIFYING PROTEIN NUCLEAR TRANSPORT USING THE SAME

PROBES FOR DETECTING PROTEIN NUCLEAR TRANSPORT AND METHOD FOR DETECTING AND QUANTIFYING PROTEIN NUCLEAR TRANSPORT USING THE SAME 実績あり

外国特許コード F110004316
整理番号 A161-14WO
掲載日 2011年7月13日
出願国 カナダ
出願番号 2559299
公報番号 2559299
公報番号 2559299
出願日 平成17年3月9日(2005.3.9)
公報発行日 平成17年9月15日(2005.9.15)
公報発行日 平成22年8月17日(2010.8.17)
優先権データ
  • 特願2004-066424 (2004.3.9) JP
発明の名称 (英語) PROBES FOR DETECTING PROTEIN NUCLEAR TRANSPORT AND METHOD FOR DETECTING AND QUANTIFYING PROTEIN NUCLEAR TRANSPORT USING THE SAME 実績あり
発明の概要(英語)

A convenient and highly accurate method for detecting protein nuclear migration due to an endogenous or foreign substance in a local area such as a vital cell or an individual organism with the use of a pair of probes for detecting protein nuclear migration, characterized in that the probes comprising a probe I having the protein, the nuclear migration of which is to be detected and quantified, connected to either the N-terminal side or the C-terminal side of a fused structure [intein-C/labeled protein-C] wherein at least a C-terminal side polypeptide obtained by halving intein is connected to a C-terminal side polypeptide obtained by halving a labeled protein in this order,

and another probe II having a nuclear localization signal connected to either the N-terminal side or the C-terminal side of a fused structure [labeled protein-N/intein-N] wherein at least the remaining N-terminal side polypeptide obtained by halving intein is connected to the remaining N-terminal side polypeptide obtained by halving the labeled protein in this order.

  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • OZAWA, TAKEAKI,
  • UMEZAWA, YOSHIO
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Endocrine Disrupters AREA
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