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Process for producing retinal neurocyte and retinal neurocyte produced by the process

外国特許コード F110004592
整理番号 K05204CN2
掲載日 2011年7月20日
出願国 中華人民共和国
出願番号 200710196506
公報番号 101186900
公報番号 101186900
出願日 平成16年6月11日(2004.6.11)
公報発行日 平成20年5月28日(2008.5.28)
公報発行日 平成22年9月15日(2010.9.15)
優先権データ
  • 特願2003-166646 (2003.6.11) JP
発明の名称 (英語) Process for producing retinal neurocyte and retinal neurocyte produced by the process
発明の概要(英語) A method for producing retinal nerve cells by inducing differentiation of iris pigmented epithelial cells into the retinal nerve cells.
In a first method, iris pigmented epithelial cells derived from a mammal and embryo retinal stem cells derived from a bird are co-cultured.
In a second method, iris pigmented epithelial cells of a bird or a mammal is isolated, and the iris pigmented epithelial- cells is subjected to adherent culturing.
According to these methods, the retinal nerve cells can be produced by using iris pigmented epithelial cells collected from a patient per se.
Therefore, there is a possibility that highly effective regenerative medical treatment can be realized.
特許請求の範囲(英語) [claim1]
1. One method for producing retinal nerve cells, characterized in, constituting the method comprises the following steps:
The step of separating iris pigmented epithelial cells from an eyeball; and
In serum-free medium on iris pigmented epithelial cells by contacting the cultured cluster, which induce the step of differentiating into Retinal Nerve Cells.
[claim2]
2. Method for producing retinal nerve cells according to claim 1, characterized in: iris pigmented epithelial cells derived from birds or mammals.
[claim3]
3. Method for producing retinal nerve cells according to claim 1 or 2, characterized in: at the beginning of the contact medium in the culture medium without serum, for 1-100 ng/ml FGF2 molality of, FGF9, at least one of the CNTF.
[claim4]
4. According to claim 1 to 3 in the production method according to any one of the retinal nerve cells, characterized in: at the beginning of the iris pigmented epithelial cells in serum-free medium density of the cells is less than or equal to 1x105 amino/cm2.
[claim5]
5. One method for producing retinal nerve cells, characterized in, constituting the method comprises the following steps:
The step of separating iris pigmented epithelial cells from an eyeball;
And the iris pigmented epithelial cells implanted on a culture medium containing FGF2/or FGF9 and the step of culturing and initially contacts; and
After the initial contact step of culturing, FGF2/or FGF9 from the culture medium and the removed, moreover, the medium was supplemented CNTF, iris pigmented epithelial cells were cultured for making contact, which induce the step of differentiating into Retinal Nerve Cells.
[claim6]
6. Method for producing retinal nerve cells according to claim 5, characterized in: a step of culturing the initial contact in the case of the medium is serum-free medium, in the step of then gently to the retinal nerve cell differentiation, and in a medium supplemented serum.
[claim7]
7. One retinal nerve cells, characterized in: that the retinal nerve cells are produced according to claim 1 to 6 obtained by a method according to any one.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • KOSAKA MITSUKO
国際特許分類(IPC)
参考情報 (研究プロジェクト等) PRESTO Time's Arrow and Biosignaling AREA
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