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Process for producing tissue cell from pluripotent stem cell derived from iris pigment epithelial cell of animal and tissue cell obtained by the process

外国特許コード F110004593
整理番号 K05205CN
掲載日 2011年7月20日
出願国 中華人民共和国
出願番号 200480015005
公報番号 1798833
公報番号 1798833
出願日 平成16年6月10日(2004.6.10)
公報発行日 平成18年7月5日(2006.7.5)
公報発行日 平成22年6月9日(2010.6.9)
国際出願番号 JP2004008120
国際公開番号 WO2004111212
国際出願日 平成16年6月10日(2004.6.10)
国際公開日 平成16年12月23日(2004.12.23)
優先権データ
  • 2004JP008120 (2004.6.10) WO
  • 特願2003-166684 (2003.6.11) JP
発明の名称 (英語) Process for producing tissue cell from pluripotent stem cell derived from iris pigment epithelial cell of animal and tissue cell obtained by the process
発明の概要(英語) A process for producing tissue cells derived from iris pigment epithelial cells of an animal, by which problems, such as concern about immunological rejection caused by cell transplantation, ethical issues and unbalance between the demand and supply on transplant cell sources, can be solved; and tissue cells produced by the process.
In this process for producing tissue cells, first, iris pigment epithelial cells isolated from an animal eyeball are selectively cultured according to a floated coagulated mass culturing technique to thereby obtain pluripotent stem cells.
Thereafter, these pluripotent stem cells are cultured with the use of, for example, serum to thereby effect production of various tissue cells.
特許請求の範囲(英語) [claim1]
1. One method for producing tissue cells, characterized in, including the steps of:
According to a floated coagulated mass culturing method of the iris pigmented epithelial cells isolated from the eyeball of an animal are selectively cultured, thereby obtaining step of pluripotent stem cells; and
Culturing the pluripotent stem cells, pluripotent stem cells obtained from the iris pigmented epithelial.
[claim2]
2. Process for producing tissue cells according to claim 1, characterized in:
Animal is a chicken, mouse, rat, human can be omitted.
[claim3]
3. Process for producing tissue cells according to claim 1 or 2, characterized in:
Animal is a postnatal individual.
[claim4]
4. According to claim 1 to 3 according to any one of the process for producing tissue cells, characterized in:
Pluripotent stem cells are Oct-3/4 positive, and a mesodermal sex differentiation capability/or has a three.
[claim5]
5. According to claim 1 to 4 according to any one of the process for producing tissue cells, characterized in:
Iris pigmented epithelial cells are removed from the eyeball of the animal through iris tissue of the iris tissue taking-out process, iris pigmented epithelial tissue is isolated and collected from an iris of the iris pigmented epithelial separation step of separating the compound.
[claim6]
6. Process for producing tissue cells according to claim 5, characterized in, iris tissue taking-out process comprises the following stages:
The iris tissue removal stage, to excise only iris tissue from the eyeball of the animal;
Enzyme treatment stage, the excised iris tissue enzyme treatment; and
The iris-tissue-restoring stage, to carry out the iris-tissue-restoring enzyme treatment.
[claim7]
7. According to claim 1 to 6 according to any one of the process for producing tissue cells, characterized in:
From the pluripotent stem cells obtained in the step of tissue cells, in culture in differentiation conditions, causes the pluripotent stem cells to differentiate into more tissue cells 1 or 1.
[claim8]
8. Process for producing tissue cells according to claim 7, characterized in:
Under differentiating conditions used were cultured in a culture of serum.
[claim9]
9. Process for producing tissue cells according to claim 8, characterized in:
Serum is fetal calf serum or avian serum.
[claim10]
10. Process for producing tissue cells according to claim 8 or 9, characterized in:
The culture of under differentiating conditions, further use of growth factors.
[claim11]
11. Process for producing tissue cells according to claim 10, characterized in:
Growth factor is EGF or FGF.
[claim12]
12. A tissue cell, characterized in: the tissue cells are set forth in any one according to claim 1 to 11 obtained by a method producing the tissue cells.
[claim13]
13. Tissue cells according to claim 12, characterized in:
Tissue cells are ectodermal cells or cells derived from ectoderm, mesodermal cells derived from mesodermal cell or, or endodermal cells or cells derived from endoderm of either one.
[claim14]
14. Tissue cells according to claim 12 or 13, characterized in:
For forming an intravital organ tissue cells forms tissue.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • KOSAKA MITSUKO
国際特許分類(IPC)
参考情報 (研究プロジェクト等) PRESTO Time's Arrow and Biosignaling AREA
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