Method of discriminating bovine, thus discriminated bovine and kit for discriminating bovine
Disclosed is a method of discriminating a bovine individual whereby proteins participating in the economical characteristics of beef cattle are identified by a proteomic analysis and a bovine individual having useful economical characteristics is discriminating with the use of these proteins as biomarkers. Also disclosed is a kit for discriminating a bovine individual to be used therein. The method of discriminating a bovine individual as described above comprises: (1) a collection step for collecting a body tissue from a bovine
(2) an extraction step for extracting all proteins from the body tissue thus collected
(3) a detection step for detecting wild type annexin A5 protein, an isoform of the wild type annexin A5 protein and a modified protein of the wild type annexin A5 protein or the isoform thereof that are contained in the all of the extracted proteins
and (4) a discrimination step for discriminating whether or not the average carcass weight is increased depending on the detected annexin A5 types. The wild type annexin A5 protein, the isoform thereof and modified proteins of the same are detected by using two-dimensional electrophoresis or an antigen-antibody reaction.