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Process for producing tissue cell from pluripotent stem cell derived from iris pigment epithelial cell of animal and tissue cell obtained by the process

外国特許コード F110004857
整理番号 K05205KR
掲載日 2011年7月22日
出願国 大韓民国
出願番号 20057023771
公報番号 20060017542
公報番号 100797143
出願日 平成17年12月9日(2005.12.9)
公報発行日 平成18年2月23日(2006.2.23)
公報発行日 平成20年1月22日(2008.1.22)
国際出願番号 JP2004008120
国際公開番号 WO2004111212
国際出願日 平成17年12月9日(2005.12.9)
国際公開日 平成16年12月23日(2004.12.23)
優先権データ
  • 特願2003-166684 (2003.6.11) JP
発明の名称 (英語) Process for producing tissue cell from pluripotent stem cell derived from iris pigment epithelial cell of animal and tissue cell obtained by the process
発明の概要(英語) A process for producing tissue cells derived from iris pigment epithelial cells of an animal, by which problems, such as concern about immunological rejection caused by cell transplantation, ethical issues and unbalance between the demand and supply on transplant cell sources, can be solved; and tissue cells produced by the process.
In this process for producing tissue cells, first, iris pigment epithelial cells isolated from an animal eyeball are selectively cultured according to a floated coagulated mass culturing technique to thereby obtain pluripotent stem cells.
Thereafter, these pluripotent stem cells are cultured with the use of, for example, serum to thereby effect production of various tissue cells.
特許請求の範囲(英語) [claim1]

From the eyeballs of animals [1] just how to produce cells that characterized that process get the pluripotent stem cells by IRIS pigment epithelial cells isolated culture suspended cohesive mass culture how to selectively process and these pluripotent stem cell culture and the generation of pluripotent stem cells from the tissue cells and tissues.

[2] these animals are in claim 1 characterized by niwatori, mouse, rat, and human or production methods in tissue cells.

[3] these animals are in claim 1 characterized by population after birth or 2 cells in production methods.

[4] these pluripotent stem cells are Oct ― to be 3/4 positive and / or 3 endoderm differentiation that, claims to feature 1 any of the 3 mentioned in paragraph (1) of production methods of tissue cells.

[5] above the IRIS pigment epithelium, and so the IRIS organization was enough to remove the IRIS tissue from the eye of the animal,

And IRIS pigment epithelial separation process to separate the IRIS pigment epithelium from above the IRIS tissue excised than just claim to be released 1 4 any force 4 paragraphs in the tissue cells in production methods.

[6] above the IRIS tissue removal process the IRIS Organization cut except stage to remove only the IRIS tissue from the eye of the animal and

Enzymatic treatment of the tumor above the IRIS tissue enzyme processing stages and

To claim 5 characterized to include IRIS tissue recovery process to restore the IRIS tissue treated with these enzymes and cells in production methods.

Claims process [7] these pluripotent stem cells from the tissue cells, these pluripotent stem cells, differentiation under culture, to one or more types of tissue cells differentiation to feature 1 in 6 in force paragraph 1 production methods in tissue cells.

To claim 7 features to do the culture using serum [8] above differentiation under cultivation, production method of tissue cells.

[9] These serum ushi fetal serum or serum Tri that characterized a claim 8, method for production of cells in tissues.

[10] above differentiation under culture using growth factors in addition to features claims

8 or 9 cells in production methods.

[11] these growth factors will claim 10 to feature that the EGF or FGF, method for production of cells in tissues.

[12] claim 1 11 any power, (1) how to produce cells of obtained tissue cells

[13] these cells are outside Ectodermal cells or endoderm cells and mesodermal cell or in Ectodermal cells, in mesodermal cells or claim 12 to feature in any of the mesodermal cells tissue cells in.

[14] these tissue cells to form tissues make up the organs of the human body to claim 12 or 13 in tissue cells.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • KOSAKA MITSUKO
国際特許分類(IPC)
参考情報 (研究プロジェクト等) PRESTO Time's Arrow and Biosignaling AREA
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