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METHOD OF QUICKLY DETECTING ANTIGEN BY FLUORESCENCE CORRELATION SPECTROMETRY

外国特許コード F110004908
整理番号 RX01P04WO
掲載日 2011年7月25日
出願国 大韓民国
出願番号 20067027538
公報番号 20070026671
公報番号 100847349
出願日 平成18年12月28日(2006.12.28)
公報発行日 平成19年3月8日(2007.3.8)
公報発行日 平成20年7月21日(2008.7.21)
優先権データ
  • 特願2004-166440 (2004.6.3) JP
発明の名称 (英語) METHOD OF QUICKLY DETECTING ANTIGEN BY FLUORESCENCE CORRELATION SPECTROMETRY
発明の概要(英語)

It is intended to provide a detection and/or assay method whereby an abnormal prion or an antigen, for example, an antigenic protein such as a harmful protein contained in a food material can be quickly and accurately detected and/or assayed by a convenient procedure. In detecting or assaying an antigen molecule by using the fluorescence correlation spectrometry (FCS), a fluorescence- labeled antibody fragment and a non-fluorescence-labeled complete antibody capable of binding to the fluorescence-labeled antibody fragment via an antigen are employed. Thus, a significant difference in diffusion speed arises between the fluorescence-labeled antibody fragment not bonded to the antigen and a complex formed by the antigen/antibody reaction among the fluorescence-labeled antibody fragment, the antigen and the non-fluorescence-labeled complete antibody.

According to this method, an antigen can be detected and assayed by using FCS, even in the case of an antigen with a relatively low molecular weight such as an antigenic protein, independently from the shape or molecular weight of the antigen and thus antigens over a wide scope can be quickly assayed. TM KIPO & WIPO 2007

  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • KINJO MASATAKA,
  • HORIUCHI MOTOHIRO,
  • FUJII FUMIHIKO,
  • SAKATA HIROSHI,
  • TAMURA MAMORU,
  • UENO MASAYOSHI,
  • YANAGIYA TAKAYUKI
国際特許分類(IPC)
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