TOP > 外国特許検索 > Method of forming normal regenerated tissue, the normal regenerated tissue, and method of calibrating sensitivity and so on

Method of forming normal regenerated tissue, the normal regenerated tissue, and method of calibrating sensitivity and so on

外国特許コード F110004932
整理番号 Y0223WO
掲載日 2011年7月25日
出願国 大韓民国
出願番号 20047005511
公報番号 20050036855
公報番号 100777993
出願日 平成16年4月14日(2004.4.14)
公報発行日 平成17年4月20日(2005.4.20)
公報発行日 平成19年11月28日(2007.11.28)
国際出願番号 JP2002010674
国際公開番号 WO2003039611
国際出願日 平成16年4月14日(2004.4.14)
国際公開日 平成15年5月15日(2003.5.15)
優先権データ
  • 特願2001-317502 (2001.10.15) JP
発明の名称 (英語) Method of forming normal regenerated tissue, the normal regenerated tissue, and method of calibrating sensitivity and so on
発明の概要(英語) A normal regenerated tissue is formed by exposing to radiation a binding tissue or a supporting tissue originating in an organ to thereby form a feeder layer and then transplanting epithelial cells thereon to form a layered structure.
By conveniently and surely providing a regenerated tissue by the three-dimensional culture with the use of a human-origin normal tissue as a base, it is possible to construct systems for calibrating effects and side effects of chemicals such as drugs or calibrating sensitivities thereof with the use of regenerated tissues as models of corresponding tissues respectively.
特許請求の範囲(英語) [claim1]
1. Organs derived from connective tissue, the cells or support organization irradiation Friday layer is formed, and then planted the epithelial cells this is layered formation of normal tissues to be characterized.
[claim2]
2. Formation of connective tissue or its constituent cells or support organization and claim 1 characterized that epithelial cells derived from organs of normal regeneration method.
[claim3]
3. Connective tissue, the cells or support organizations, organ-derived fibroblasts, endothelial cells and those of at least in at least one. formation of normal tissues and advanced payment to claim 1 or 2.
[claim4]
4. Later planted fibroblast cells from the organ on vascular endothelial cells in medium then radiation, Vida 1 layer is formed, then the epithelial cells planted, this heavy layer formation of normal tissues or to combine claims 1 to 3.
[claim5]
5. After planting the epithelial cells to extracellular by adding one or more of the matrix, epithelial cell layer claim 1 characterized to and not 4 any formation of normal tissues.
[claim6]
6. Extracellular matrix, extracellular formation of claim 5 to feature the substrate structures or adhesion molecules that successfully regenerated tissue.
[claim7]
7. Extracellular matrix is the formation of normal tissues that characterized by 1 or more of the following: collagen, elastin, proteog licang, EAE, laminin, and Tennesse Tin claim 5 or 6.
[claim8]
8. Addition to collagen or collagen and five ronectin and laminin after planting the epithelial cells and epithelial cell layer forming method of normal tissues combine to claim 7.
[claim9]
9. Sharing of connective tissue, the cells or support organization are organs derived from different cells and its culture supernatant at least and at least one formation of normal tissues of either claim 1 characterized by exposure to radiation after a coculture on both, to form a Vida 1 layer or 8
[claim10]
10. from sympatric with other cells, epithelial cells,. thereof and from of of culture supernatants of small than by coculture with one layered formation of normal tissues in one of 9 claim 1 and the special payment to.
[claim11]
11. claim 1 and the special payment that the radiation X rays or? c? c line no 1 formation of normal tissues or 0.
[claim12]
12. carbon dioxide concentrations 5-1 atmospheric concentration of 5%, 8 5-9 5% 2 and then 0-4 0 ^ of epithelial cells cultured in the temperature range, layer to combine claims 1 or 1 method for forming one of the normal tissues.

1 3. (PVC figure) derleyahoo from the connective tissue of organs or cells or support organization in stratified epithelial cell layer was having features shall have normal tissues. ―
[claim13]
14. · Epithelial cell layer, nerve, oral mucosa, skin, bronchial, mammary gland, liver, or claim 1 and the special payment that the kidney came from 3 health regeneration.
[claim14]
15. claim 1 characterized that connective tissue or its constituent cells or support tissue and epithelial cells derived from organs of 3 or 1 4 health regeneration.
[claim15]
16. connective tissue, the cells or support organizations, organ-derived fibroblasts, endothelial cells and organization of those small than in any that claim 1 and the special payment to 3 or 1 normal tissues of one or more of the 5
[claim16]
17. Vida 1 layer layer is configured through the upper layer of fibroblasts derived from the organs and vascular endothelial cells and the FIDA 1 layer 1 layer on a heavy layer placing epithelial cells was enhanced to normal tissues.
[claim17]
18. epithelial cells is about 4 layers or more heavy layer claim 1 and the characteristics that have made 3 or 1 of 7 health regeneration.
[claim18]
19. claim 1 3 or 1 normal tissues and advanced payment to one of the 8 health regeneration is placed on the Board.
[claim19]
20. touching the normal regeneration medium or medium, and distribution are who claim 1 characterized that 3 or 1 normal tissues of one or more of the 9.
[claim20]
21. claims 1 9 or two is more than one of the normal tissues of 0 medium also is normal regeneration to be passed in the distribution channel of the nutrient solution.
[claim21]
22. claims 1 3 or 2 play 1 normal tissues organs model body Defen... It makes sense to normal tissues.
[claim22]
23. claim 1 3 or 2 that inhibits the growth of regenerated epithelial cells after the AA light radiation to normal tissues in one of two heavy layer test for susceptibility test of the susceptibility of chemical additives, irradiated ί strands cancer cells that planted the cancer cells in the epithelial cell layer was to feature.
[claim23]
24. low oxygenation of claims 2 to test flight characteristics and 3 susceptibility test method.
[claim24]
25. after planting a cancer cell to collagen and other extracellular and fill one or more of the matrix and collagen and other extracellular claim 2 planted cancer cells after supplemented with one or more of the matrix, followed by the test for susceptibility to feature 3 or 2 4 susceptibility test methods.
[claim25]
26. claim 1 3 or 2 that inhibits the growth of regenerated epithelial cells after the AA light radiation to normal tissues in one of two layered planted cancer cells in the epithelial cell layer was collagen and other extracellular replenished more than 1 species of the matrix and testing method of blood vessel angiogenesis planted on vascular endothelial cells, caused by cancer cells vascular angiogenic ability tests and the addition of chemicals to feature.
[claim26]
27. claim 1 3 and 2 planted the cancer cells in epithelial cells overlap after the AA light radiation to normal tissues in one of two, that inhibits the growth of regenerated epithelial cells, collagen and other extracellular test methods of transition characterized by whole upside-down to put one or more of the matrix, metastasis or infiltration test or infiltration.
[claim27]
28. claim 1 3 or 2 characteristics and susceptibility tests by addition of chemical and radiation sensitivity of normal tissues, whereas normal tissues in one of two test methods.
[claim28]
29. claim 1 3 or 2 gene transfer to test efficiency of genetic child adoption organizations or 2 normal play to feature test method.
[claim29]
30.-radiation irradiation to inhibit growth of regenerative epithelium cells claim 2 or o line in the x-ray to feature 3 or 2 9 ways.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • NAKATSUGAWA SHIGEKAZU
国際特許分類(IPC)
ライセンスをご希望の方、特許の内容に興味を持たれた方は、問合せボタンを押してください。

PAGE TOP

close
close
close
close
close
close