TOP > 外国特許検索 > Bacterial cell component-unresponsive model mouse

Bacterial cell component-unresponsive model mouse 実績あり

外国特許コード F110005028
整理番号 A031-19US
掲載日 2011年8月18日
出願国 アメリカ合衆国
出願番号 88932400
公報番号 7078585
出願日 平成12年1月13日(2000.1.13)
公報発行日 平成18年7月18日(2006.7.18)
国際出願番号 JP2000000132
国際公開番号 WO2000041561
国際出願日 平成12年1月13日(2000.1.13)
国際公開日 平成12年7月20日(2000.7.20)
優先権データ
  • 特願1999-007365 (1999.1.14) JP
  • 特願1999-228282 (1999.8.12) JP
  • 特願1999-309238 (1999.10.29) JP
  • 2000WO-JP00132 (2000.1.13) WO
発明の名称 (英語) Bacterial cell component-unresponsive model mouse 実績あり
発明の概要(英語) (US7078585)
A knockout mouse which is unresponsive to peptidoglycan, a lipoprotein/lipopeptide and the like, and is useful for elucidating the contribution of individual members of the TLR family to a signaling stimulated with bacterial cell components in vivo, in particular, the role of TLR2 and MyD88 in vivo.
A bacterial cell component-unresponsive knockout mouse is generated by a process comprising the steps of: a targeting vector is constructed by replacing a whole or a part of a gene fragment of an exon region containing a cytoplasmic region of TLR2 or MyD88 gene and the like with a plasmid having a poly A signal and a marker gene; the targeting vector is introduced into an embryonic stem cell; the targeting embryonic stem cell having a homologously recombined TLR2 or MyD88 gene is microinjected into the blastocyst of a mouse and the blastocyst is put back into the uterus of a recipient mouse.
特許請求の範囲(英語) [claim1]
1. A mouse comprising homozygous disruption of TLR2 gene in its genome, wherein such disruption results in no production of endogenous TLR2 protein, and wherein said mouse exhibits the phenotype of being unresponsive to bacterial cell component(s) that is a lipoprotein/lipopeptide.
[claim2]
2. The mouse according to claim 1, wherein a lipoprotein/lipopeptide is a macrophage-activating lipopeptide obtained from bacteria which belong to Mycoplasma.
[claim3]
3. The mouse according to claim 1 that is further unresponsive to peptidoglycan as a bacterial cell component.
[claim4]
4. The mouse according to claim 1 that is further hyporesponsive to a cell wall fraction of Gram-positive bacteria.
[claim5]
5. A mouse comprising homozygous disruption of MyD88 gene in its genome, wherein such disruption results in no production of endogenous MyD88 protein, and wherein said mouse exhibits the phenotype of being unresponsive to bacterial cell component(s) that is a lipoprotein/lipopeptide.
[claim6]
6. The mouse according to claim 5, wherein a lipoprotein/lipopeptide is a macrophage-activating lipopeptide obtained from bacteria which belong to Mycoplasma.
[claim7]
7. The mouse according to claim 5 that is further unresponsive to peptidoglycan as a bacterial cell component.
[claim8]
8. The mouse according to claim 5 that is further unresponsive to a cell wall fraction of Gram-positive bacteria.
[claim9]
9. The mouse according to claim 5 that is further unresponsive to endotoxin as a bacterial cell component.
[claim10]
10. The mouse according to claim 5 that is further unresponsive to lipoteichoic acid as a bacterial cell component.
[claim11]
11. The mouse according to claim 5 that is further unresponsive to Mycobacterium tuberculosis lysate as a bacterial cell component.
  • 発明者/出願人(英語)
  • AKIRA SHIZUO
  • TAKEUCHI OSAMU
  • TAKEDA KIYOSHI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 800/18
  • 800/3
参考情報 (研究プロジェクト等) CREST Host Defense Mechanism AREA
ライセンスをご希望の方、特許の内容に興味を持たれた方は、問合せボタンを押してください。

PAGE TOP

close
close
close
close
close
close