TOP > 外国特許検索 > Electrochemical detection method of complementarity to nucleic acid bases

Electrochemical detection method of complementarity to nucleic acid bases

外国特許コード F110005204
整理番号 A161-03US
掲載日 2011年8月25日
出願国 アメリカ合衆国
出願番号 47165701
公報番号 20050153285
公報番号 7169614
出願日 平成13年3月13日(2001.3.13)
公報発行日 平成17年7月14日(2005.7.14)
公報発行日 平成19年1月30日(2007.1.30)
国際出願番号 JP2001001974
国際公開番号 WO2002073183
国際出願日 平成13年3月13日(2001.3.13)
国際公開日 平成14年9月19日(2002.9.19)
優先権データ
  • 2001WO-JP01974 (2001.3.13) WO
発明の名称 (英語) Electrochemical detection method of complementarity to nucleic acid bases
発明の概要(英語) (US7169614)
A convenient and commonly applicable method for the specific detection of a nucleic acid with an arbitrary sequence is provide.
This method comprises attaching at least a nucleic acid single strand to an electrode, bringing the thus-obtained modified electrode in contact with a solution containing the analyte single-stranded nucleic acid, and measuring the redox reaction of the redox marker.
特許請求の範囲(英語) [claim1]
1. A method for electrochemical detection of nucleobase pair complementarity, which comprises: contacting a modified electrode obtained by bonding at least a single stranded nucleic acid to an electrode so that the single-stranded nucleic acid is oriented vertically with respect to the surface of the electrode and the electrode is a gold electrode, with a solution containing an analyte single stranded nucleic acid and a redox marker, wherein the redox marker is [Mo (CN)6]4/13- or [Fe (CN)6]4/13-;
and
detecting the disappearance of an electric signal generated by reaction between the modified electrode and the redox marker, when the single stranded nucleic acid on the modified electrode and the analyte single stranded nucleic acid are complementary.
[claim2]
2. The method for electrochemical detection of nucleobase pair complementarity of claim 1, wherein the electrode is a gold disk electrode.
[claim3]
3. The method for electrochemical detection of nucleobase pair complementarity of claim 1, wherein a molecule that is not complementary to the analyte single stranded nucleic acid is bonded to the modified electrode along with the single stranded nucleic acid for vertically orienting the single stranded nucleic acid with respect to the surface of the electrode.
[claim4]
4. The method for electrochemical detection of nucleobase pair complementarity of claim 3, wherein the single stranded nucleic acid and the molecule that is not complementary to the analyte single stranded nucleic acid form a mixed monolayer on the surface of the electrode.
[claim5]
5. The method for electrochemical detection of nucleobase pair complementarity of claim 3, wherein the molecule that is not complementary to the analyte single stranded nucleic acid is 6-mercapto-1-hexanol.
[claim6]
6. The method for electrochemical detection of nucleobase pair complementarity of claim 1, wherein the single stranded nucleic acid is a single stranded peptide nucleic acid.
[claim7]
7. The method for electrochemical detection of nucleobase pair complementarity of claim 1, wherein the concentration of the analyte single stranded nucleic acid is 10-11 to 10-2 M.
[claim8]
8. The method for electrochemical detection of nucleobase pair complementarity of claim 1, wherein the analyte single stranded nucleic acid is a single stranded DNA.
[claim9]
9. The method for electrochemical detection of nucleobase pair complementarity of claim 1, wherein the redox marker is [Fe(CN)6]4-/3-.
[claim10]
10. The method for electrochemical detection of nucleobase pair complementarity of claim 9, wherein the concentration of [Fe(CN)6]4-/3- is 0.1 to 1 mM.
[claim11]
11. The method for electrochemical detection of nucleobase pair complementarity of claim 1, wherein the redox reaction is measured by voltammetry.
[claim12]
12. A modified electrode for detecting nucleobase pair complementarity by the method of claim 1, which comprises a mixed monolayer of a peptide nucleic acid and a molecule that is not complementary to the analyte nucleic acid, formed on the surface of a gold disk electrode.
  • 発明者/出願人(英語)
  • UMEZAWA YOSHIO
  • AOKI HIROSHI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 436/94
  • 204/527
  • 210/746
  • 422/98
  • 435/6.11
  • 435/287.2
  • 536/23.1
参考情報 (研究プロジェクト等) CREST Endocrine Disrupters AREA
ライセンスをご希望の方、特許の内容に興味を持たれた方は、問合せボタンを押してください。

PAGE TOP

close
close
close
close
close
close