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Process for producing nerve stem cells, motor neurons, and GABAergic neurons from embryonic stem cells 新技術説明会 実績あり

外国特許コード F110005211
整理番号 A192-02US
掲載日 2011年8月26日
出願国 アメリカ合衆国
出願番号 47249001
公報番号 20040092012
公報番号 7294510
出願日 平成13年10月3日(2001.10.3)
公報発行日 平成16年5月13日(2004.5.13)
公報発行日 平成19年11月13日(2007.11.13)
国際出願番号 JP2001008703
国際公開番号 WO2002081663
国際出願日 平成13年10月3日(2001.10.3)
国際公開日 平成14年10月17日(2002.10.17)
優先権データ
  • 特願2001-099074 (2001.3.30) JP
  • 2001WO-JP08703 (2001.10.3) WO
発明の名称 (英語) Process for producing nerve stem cells, motor neurons, and GABAergic neurons from embryonic stem cells 新技術説明会 実績あり
発明の概要(英語) (US7294510)
The present invention provides a method for producing motor neurons and GABAergic neurons characterized by including suspension-culturing embryonic stem cells in the presence or absence of a protein noggin to form embryoid bodies, selectively amplifying into neural stem cells from them by suspension culture in the presence of a fibroblast growth factor and a sonic hedgehog protein, and then differentiating the same.
According to this method, at least motor neurons and GABAergic neurons can be systemically and efficiently produced from ES cells.
Selective acquisition of neurons would be applicable to transplant therapy for amyotrophic lateral sclerosis, Huntington's chorea, Alzheimer's disease, etc.
特許請求の範囲(英語) [claim1]
1. A method for forming embryoid bodies, which comprises subjecting embryonic stem cells to suspension culture in the presence of a noggin protein, wherein said noggin protein is added to the culture prior to formation of embryoid bodies.
[claim2]
2. A method for producing neural stem cells, which comprises subjecting embryonic stem cells to suspension culture in the presence of a noggin protein, to thereby form embryoid bodies, and subsequently subjecting the embryoid bodies to suspension culture in the presence of a fibroblast growth factor and a sonic hedgehog protein to thereby form a culture of neural stem cells.
[claim3]
3. The method according to claim 2, wherein concentration of the fibroblast growth factor in culture medium is 5 to 50 ng/mL, and that of the sonic hedgehog protein in culture medium is 1 to 20 nM.
[claim4]
4. A method for producing motor neurons and GABAergic neurons, which comprises subjecting ES cells to suspension culture in the presence of a noggin protein, to thereby form embryoid bodies, and subsequently subjecting the embryoid bodies to suspension culture in the presence of a fibroblast growth factor and a sonic hedgehog protein, to thereby induce formation of neural stem cells, and differentiating the resultant neural stem cells in a differentiation medium.
[claim5]
5. The method according to claim 4, wherein concentration of the fibroblast growth factor in culture medium is 5 to 50 ng/mL, and that of the sonic hedgehog protein in culture medium is 1 to 20 nM.
[claim6]
6. The method according to claim 4, wherein the resultant neurons are substantially formed of motor neurons and GABAergic neurons.
[claim7]
7. The method according to claim 5, wherein the resultant neurons are substantially formed of motor neurons and GABAergic neurons.
[claim8]
8. The method according to claim 2, wherein the culture of neural stem cells comprises neurospheres.
  • 発明者/出願人(英語)
  • OKANO HIDEYUKI
  • SHIMAZAKI TAKUYA
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 435/377
  • 435/325
  • 435/354
  • 435/365
  • 435/368
  • 530/399
参考情報 (研究プロジェクト等) CREST Development, Differentiation, and Regeneration in Biological Systems AREA
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