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Gene carriers with the use of polysaccharide and process for producing the same 実績あり

外国特許コード F110005347
整理番号 K01202US
掲載日 2011年8月31日
出願国 アメリカ合衆国
出願番号 47136602
公報番号 20060084149
公報番号 7521433
出願日 平成14年3月11日(2002.3.11)
公報発行日 平成18年4月20日(2006.4.20)
公報発行日 平成21年4月21日(2009.4.21)
国際出願番号 JP2002002228
国際公開番号 WO2002072152
国際出願日 平成14年3月11日(2002.3.11)
国際公開日 平成14年9月19日(2002.9.19)
優先権データ
  • 特願2001-069655 (2001.3.13) JP
  • 特願2001-130705 (2001.4.27) JP
  • 2002WO-JP02228 (2002.3.11) WO
発明の名称 (英語) Gene carriers with the use of polysaccharide and process for producing the same 実績あり
発明の概要(英語) (US7521433)
Disclosed are gene carriers utilizing beta-1,3-glucan and methods of preparing the same.
The beta-1,3-glucan has at least one 1,6-glucopyranoside branch and is chemically modified through periodate oxidation and reductive amination so as to impart nucleic acid-binding functional groups (for example, cationic functional groups) to at least some of the 1,6-glucopyranoside branches thereof.
Triple helix beta-1,3-glucan is dissolved in a polar organic solvent to form single-stranded beta-1,3-glucan.
By replacing, in the presence of a nucleic acid, the polar organic solvent for the solution containing the chemically modified single-stranded beta-1,3-glucan by water, a complex (gene carrier) is formed in which the nucleic acid is bound to double-stranded beta-1,3-glucan.
特許請求の範囲(英語) [claim1]
1. A gene carrier composed of a complex in which a single-stranded nucleic acid is bound to a double-stranded beta -1,3-glucan wherein the beta -1,3-glucan has at least one 1,6-glucopyranoside branch per repeating unit of polysaccharide and is selected from schizophyllan, lentinan, grifolan, and scleroglucan, and wherein at least some of 1,6-glucopyranoside branches are chemically modified to be functionalized with nucleic acid-binding functional groups which are cationic functional groups formed by reaction of the polysaccharide with chain or cyclic compounds containing at least one primary or secondary amino group, wherein the chemical modification is carried out by periodate-oxidizing the 1,6-glucopyranoside branches and then reductive-aminating the periodated 1,6-glucopyranoside branches to impart the nucleic acid binding functional group thereto, and wherein the 1,6-glucopyranoside branch which has been functionalized with the nucleic acid-binding groups is expressed by the following general formula (1):
wherein the two X's are the cationic functional groups and are identical or different.
[claim2]
2. The gene carrier of claim 1, wherein the nucleic acid-binding functional groups are introduced at a rate of at least 0.1 mol % and up to 50 mole % based on the repeating unit of the polysaccharide.
[claim3]
3. The gene carrier of claim 1, wherein the beta -1,3-glucan has a molecular weight of at least 2000.
[claim4]
4. The gene carrier of claim 1, wherein the nucleic acid is an antisense DNA.
[claim5]
5. The gene carrier of claim 1, wherein the beta -1,3-glucan comprises schizophyllan.
  • 発明者/出願人(英語)
  • KIMURA TARO
  • MIZU MASAMI
  • SAKURAI KAZUO
  • SHINKAI SEIJI
  • KOUMOTO KAZUYA
  • FUKUOKA PREFECTURAL GOVERNMENT
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • MITSUI SUGAR
国際特許分類(IPC)
米国特許分類/主・副
  • 514/54
  • 536/123.12
参考情報 (研究プロジェクト等) PRESTO Organization and Function AREA
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