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Fluorescent sensor for phosphate ion and phosphorylated peptide 実績あり

外国特許コード F110005348
整理番号 K01205US
掲載日 2011年8月31日
出願国 アメリカ合衆国
出願番号 50505603
公報番号 20050148086
公報番号 7521250
出願日 平成15年1月27日(2003.1.27)
公報発行日 平成17年7月7日(2005.7.7)
公報発行日 平成21年4月21日(2009.4.21)
国際出願番号 JP2003000705
国際公開番号 WO2003071280
国際出願日 平成15年1月27日(2003.1.27)
国際公開日 平成15年8月28日(2003.8.28)
優先権データ
  • 特願2002-045846 (2002.2.22) JP
  • 2003WO-JP00705 (2003.1.27) WO
発明の名称 (英語) Fluorescent sensor for phosphate ion and phosphorylated peptide 実績あり
発明の概要(英語) (US7521250)
Disclosed is a fluorescent sensor for phosphate ion and phosphorylated peptide, which comprises a phosphate anion-selective fluorescent compound expressed by the following general formula (1).
In the formula (1), FL represents a fluorescent functional group or atomic group having an aromatic ring or heterocyclic ring (for example, dimethylanthlyl group), and X represents a functional group or atomic group which will be liberated in an aqueous solution to form an anion (for example, NO3):
特許請求の範囲(英語) [claim1]
1. A method comprising: in an aqueous solution, providing a fluorescent sensor for phosphate ion and phosphorylated peptide, which sensor comprises a phosphate anion-selective fluorescent compound expressed by the following general formula (1):
wherein FL represents a fluorescent functional group or atomic group having an aromatic ring or heterocyclic ring, and X represents a functional group or atomic group which is liberated in an aqueous solution to form an anion;
and
sensing at least one of phosphate ion and phosphorylated peptide in the aqueous solution as a change in fluorescent intensity, wherein said phosphorylated peptide is composed of an amino acid sequence containing a hydrophobic amino acid and an anionic amino acid in addition to a phosphorylated amino acid.
[claim2]
2. The method of claim 1, wherein FL is selected from one of (a), (b), (c) and (d) in the following (2):
wherein R represents hydrogen atom, an alkyl group having 1 to 4 carbon atoms or benzyl group in the formula (d).
[claim3]
3. The method of claim 1, wherein X is NO3, a halogen atom or ClO4.
[claim4]
4. The method of claim 2, wherein X is NO3, a halogen atom or ClO4.
[claim5]
5. The method of claim 1, wherein said phosphorylated peptide exhibits a change in fluorescence corresponding to a change in concentration of the phosphorylated peptide and said method includes determining a concentration of said phosphorylated peptide based on said change in fluorescence.
[claim6]
6. An aqueous solution comprising: a fluorescent sensor which comprises a phosphate anion-selective fluorescent compound expressed by the following general formula (1):
wherein FL represents a fluorescent functional group or atomic group having an aromatic ring or heterocyclic ring, and X represents a functional group or atomic group which is liberated in the aqueous solution to form an anion;
and
a phosphate ion or phosphorylated peptide in sufficient amount to produce a fluorescence change, wherein said phosphorylated peptide is composed of an amino acid sequence containing a hydrophobic amino acid and an anionic amino acid in addition to a phosphorylated amino acid.
[claim7]
7. The solution of claim 6, wherein FL is selected from one of (a), (b), (c) and (d) in the following (2):
wherein R represents hydrogen atom, an alkyl group having 1 to 4 carbon atoms or benzyl group in the formula (d).
[claim8]
8. The solution of claim 6, wherein X is NO3, a halogen atom or ClO4.
[claim9]
9. The solution of claim 7, wherein X is NO3, a halogen atom or ClO4.
[claim10]
10. A method comprising: in an aqueous solution containing a phosphorylated peptide, providing a fluorescent sensor which comprises a compound expressed by the following general formula (1):
wherein FL represents a fluorescent functional group or atomic group having an aromatic ring or heterocyclic ring, and X represents a functional group or atomic group which is liberated in an aqueous solution to form an anion;
and
sensing an exhibited change in fluorescent intensity of the solution corresponding to a change in concentration of the phosphorylated peptide in the aqueous solution.
  • 発明者/出願人(英語)
  • HAMACHI ITARU
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 436/86
  • 435/40.5
  • 436/87
  • 436/172
参考情報 (研究プロジェクト等) PRESTO Organization and Function AREA
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