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Method or apparatus for recovering micromaterial

外国特許コード F110005365
整理番号 K01501US
掲載日 2011年9月5日
出願国 アメリカ合衆国
出願番号 50863903
公報番号 20050208465
公報番号 7824854
出願日 平成15年1月14日(2003.1.14)
公報発行日 平成17年9月22日(2005.9.22)
公報発行日 平成22年11月2日(2010.11.2)
国際出願番号 JP2003000231
国際公開番号 WO2003095088
国際出願日 平成15年1月14日(2003.1.14)
国際公開日 平成15年11月20日(2003.11.20)
優先権データ
  • 特願2002-131656 (2002.5.7) JP
  • 2003WO-JP00231 (2003.1.14) WO
発明の名称 (英語) Method or apparatus for recovering micromaterial
発明の概要(英語) (US7824854)
A substance (for example, methylcellulose) that undergoes a reversible gel to sol phase transition is utilized in a recovery method for specific micromaterials such as DNA molecules, cells, microorganisms and the like, approximately several μm in size, from a mixture containing other micromaterials, and a micromaterial is accurately and conveniently recovered.
The present invention is a method of recovering a desired micromaterial in a system comprising a medium that undergoes a reversible phase transition between a sol and a gel, a support material and micromaterials, comprising the steps of converting the medium surrounding the micromaterial to be recovered into a gel locally to immobilize said micromaterial on said support along with the gelled medium, removing the medium and micromaterials not immobilized on said support material and converting the gelled medium to a sol to recover the micromaterial.
特許請求の範囲(英語) [claim1]
1. A method of recovering a desired micromaterial from a mixture of micromaterials: comprising: providing a system comprising a medium that undergoes a reversible phase transition between a sol and a gel, a support material and a mixture of micromaterials, converting a portion of the medium surrounding the desired micromaterial into a gel locally to immobilize said desired micromaterial on said support material along with the gelled portion of the medium, removing the medium and micromaterials that are not immobilized on said support material, and converting the gelled medium to a sol to recover the desired micromaterial.
[claim2]
2. The method as in claim 1 wherein the medium has a sol to gel phase transition temperature, forms a gel when heated to at or above said phase transition temperature and forms a sol when cooled to at or below said phase transition temperature.
[claim3]
3. The method as in claim 2 wherein the phase transition temperature is from room temperature to 90 deg. C.
[claim4]
4. The method as in claim 2 wherein the medium is a water soluble cellulose derivative or a solution thereof.
[claim5]
5. The method as in claim 4 wherein the cellulose derivative is methylcellulose.
[claim6]
6. The method as in claim 1 wherein the support material is transparent.
[claim7]
7. The method as in claim 6 wherein the support material is a transparent electrode.
[claim8]
8. The method as in claim 1 wherein the desired micromaterial has a marker for identification.
[claim9]
9. The method as in claim 1 wherein the desired micromaterial is a DNA molecule, a cell or a microorganism.
[claim10]
10. The method as in claim 2 wherein the medium is heated to a temperature at or above the phase transition temperature by applying infrared irradiation and the medium is cooled to a temperature at or below the phase transition temperature by terminating the infrared irradiation.
[claim11]
11. The method as in claim 10 wherein the medium is heated using an infrared laser.
[claim12]
12. The method as in claim 11 wherein the infrared laser is a YAG laser or an Nd:YVO4 laser.
[claim13]
13. The method as in claim 3 wherein the medium is a water soluble cellulose derivative or a solution thereof.
[claim14]
14. The method as in claim 3 wherein the support material is transparent.
[claim15]
15. The method as in claim 2 wherein the desired micromaterial has a marker for identification.
[claim16]
16. The method as in claim 15 wherein the medium is heated to a temperature at or above the phase transition temperature by applying infrared irradiation and the medium is cooled to a temperature at or below the phase transition temperature by terminating the infrared irradiation.
[claim17]
17. The method as in claim 2 wherein the desired micromaterial is a DNA molecule, a cell or a microorganism.
[claim18]
18. The method as in claim 17 wherein the medium is heated to a temperature at or above the phase transition temperature by applying infrared irradiation and the medium is cooled to a temperature at or below the phase transition temperature by terminating the infrared irradiation.
[claim19]
19. A method of recovering a target micromaterial from a mixture of micromaterials, comprising providing a system comprising a medium that undergoes a reversible phase transition between a sol and a gel, a support material and a mixture of micromaterials, forming the medium into a sol which contains a mixture of micromaterials, examining the sol to identify and select at least one target micromaterial, converting a portion of the medium surrounding the target micromaterial into a gel locally to immobilize said target micromaterial on said support material along with the gelled portion of the medium, removing the medium and micromaterials that are not immobilized on said support material and converting the gelled medium to a sol to recover the target micromaterial.
  • 発明者/出願人(英語)
  • ARAI FUMIHITO
  • FUKUDA TOSHIO
  • ICHIKAWA AKIHIKO
  • KATSURAGI TOHORU
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 435/6.12
  • 435/283.1
  • 435/287.1
  • 435/287.9
参考情報 (研究プロジェクト等) PRESTO Interaction and Intelligence AREA
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