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MUTANT FOR INCREASING OLEIC ACID CONTENT OF SOYBEAN OIL AND FAT, AND RESPONSIBLE GENE THEREFOR

外国特許コード F110005415
整理番号 S2009-0506-C0
掲載日 2011年9月6日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2010JP060928
国際公開番号 WO 2010150901
国際出願日 平成22年6月21日(2010.6.21)
国際公開日 平成22年12月29日(2010.12.29)
優先権データ
  • 特願2009-147706 (2009.6.22) JP
発明の名称 (英語) MUTANT FOR INCREASING OLEIC ACID CONTENT OF SOYBEAN OIL AND FAT, AND RESPONSIBLE GENE THEREFOR
発明の概要(英語) A soybean having an oleic acid content exceeding that of the high-oleic-acid soybeans developed thus far is provided without using gene recombination. A soybean species containing a mutant gene in which the function of the GmFAD2-1b gene has been deleted, and a mutant gene in which the function of the GmFAD2-1b gene has been deleted are also provided.
特許請求の範囲(英語) [claim1]

(Claims machine translated from Japanese)
[claim2]
1. Functional deficiency type mutation was introduced the .omega.-6 fatty acid unsaturated conversion enzyme into the GmFAD2-1b gene and the GmFAD2-1a gene which the cord/code are done, the non gene recombination type oleic acid high content soy bean.
[claim3]
2. Oleic acid content, is 75% or more of the entire fatty acid quantity of the soy bean, in claim 1 the soy bean of statement.
[claim4]
3. (A) below the - (e) it possesses one feature at least, in claim 1 the soy bean of statement.
(a) The wrinkle of the epidermis of the seed it does not increase by comparison with wild type
(b) The linoleic acid of the quantity which becomes problem substantially is not formed
(c) .beta.- [kongurishinin] content in the entire reserve protein it does not decrease substantially by comparison with wild type
(d) Entire reserve protein mass it does not decrease substantially by comparison with wild type
(e) Fertility 4 where it does not decrease by comparison with wild type
[claim5]
4. The aforementioned functional deficiency type mutation, is each mutation which is selected from the group which consists of mistake sense mutation, nonsense mutation, frame shift mutation and null mutation, in claim 1 the soy bean of statement.
[claim6]
5. Functional deficiency type mutation of the aforementioned GmFAD2-1b gene, (1) the - among and below amino acid arrangements of the .omega.-6 fatty acid unsaturated conversion enzyme (8) is the mutation which it occurs in the codon which the amino acid residue which is included in the territory where from the group which consists of it is selected the cord/code is done, in claim 1 the soy bean of statement.
(1) The territory where consists of the 58th - 79th amino acid residue
(2) The territory where consists of the 88th - 108th amino acid residue
(3) The territory where consists of the 180th - 194th amino acid residue
(4) The territory where consists of the 229th - 251st amino acid residue
(5) The territory where consists of the 254th - 277th amino acid residue
(6) The territory where consists of the 109th - 114th amino acid residue
(7) The territory where consists of the 141st - 149th amino acid residue
(8) The territory 6 where consists of the 319th - 326th amino acid
[claim7]
6. residue.
The aforementioned amino acid residue, 103rd and/or is the 189th amino acid residue, in claim 5 the soy bean of statement.
[claim8]
7. The aforementioned mutation, 103rd and/or is mistake sense mutation of the codon which the 189th amino acid residue the cord/code is done, in claim 5 the soy bean of statement.
[claim9]
8. Mistake sense mutation of the codon which the aforementioned 103rd amino acid residue the cord/code is done, is something due to the substitution to the codon of the valine from the codon of glycine, in claim 7 the soy bean of statement.
[claim10]
9. Mistake sense mutation of the codon which the aforementioned 189th amino acid residue the cord/code is done, is something due to the substitution to the codon of the proline from the codon of threonine, in claim 7 the soy bean of statement.
[claim11]
10. It possesses the gene which consists of the base arrangement which is shown with arrangement number 3 or 5, in claim 5 the soy bean of statement.
[claim12]
11. Functional deficiency type mutation of the GmFAD2-1a gene, is something which is identical with the mutation of the GmFAD2-1a gene of the M23 soy bean or the KK21 soy bean, is, in claim 2 the soy bean of statement.
[claim13]
12. The KK21 soy bean where receipt number is FERM ABP-11249 and the B12 soy bean where receipt number is FERM ABP-11248 hybridization being able to point, it can, the non gene recombination type oleic acid high content soy bean.
[claim14]
13. Either of the claim 1-12 the medical supply composition which includes the soy bean or the processing ones of statement in 1 sections, the food or the fodder.
[claim15]
14. Either of the claim 1-12 the soy bean oil which in 1 sections is extracted from the soy bean or the processing ones of statement.
[claim16]
15. The soy bean oil of statement is included in claim 14, for medicine composition, for food or the material for the fodder.
[claim17]
16. Either of the claim 1-12 the fuel which in 1 sections is formed from the soy bean or the processing ones of statement.
[claim18]
17. Either of the claim 1-12 in 1 sections the soy bean of statement, or it features that the particular soy bean and other soy bean kind hybridization it can point, production method of the non gene recombination type oleic acid high content soy bean.
[claim19]
18. (I) - below (IV) each process which is chosen from the group which consists of is included, production method of the non gene recombination type oleic acid high content soy bean.
(i) The process which the soy bean kind which possesses the mutation of the GmFAD2-1a gene and the soy bean kind which possesses the mutation of the GmFAD2-1b gene hybridization it can point
(II) Administering the mutation inducement medicine or x-ray processing to the soy bean kind which possesses the functional deficiency type mutation of the GmFAD2-1a gene, the process which induces the functional deficiency type mutation of the GmFAD2-1b gene
(III) Administering the mutation inducement medicine or x-ray processing to the soy bean kind which possesses the functional deficiency type mutation of the GmFAD2-1b gene, the process which induces the functional deficiency type mutation of the GmFAD2-1a gene
(IV) Administering the mutation inducement medicine or x-ray processing to wild type soy bean kind, the process 19 which induces the functional deficiency type mutation of the GmFAD2-1b gene and the GmFAD2-1a gene
[claim20]
19. Being screening method of the non gene recombination type oleic acid high content soy bean,
(a) When either of the claim 1-12 [origonukureochidopurobu] which includes the base arrangement of the mutation region of the GmFAD2-1a gene or the GmFAD2-1b gene which the soy bean of statement has in 1 sections, and/or expanding at least one side of base arrangement and its complimentary arrangement of the aforementioned gene, as the aforementioned mutation region is included in expansion fragment, concerning the GmFAD2-1a gene or the GmFAD2-1b gene of the suffering inspection soy bean making use of [origonukureochidopuraima] which is produced expansion processing and/or the process which does hybridization processing, and
(b) The process which detects the mutation region of the aforementioned gene
It includes, the aforementioned method.
[claim21]
20. As either of the claim 1-12 included the base arrangement of the mutation region of the GmFAD2-1a gene or the GmFAD2-1b gene which the soy bean of statement has in 1 sections, [origonukureochido] which is produced.
[claim22]
21. In order 5' end of [origonukureochido] or 3' the terminal or central base, to become the mutation region of the GmFAD2-1a gene or the GmFAD2-1b gene, it is something which is produced, in claim 20 [origonukureochido] of statement.
[claim23]
22. It possesses the length of the 10-200 base, in claim 20 or 21 [origonukureochido] of statement.
[claim24]
23. Either of the claim 20-22 the microphone lower ray where [origonukureochido] of statement is locked to the backing in 1 sections.
[claim25]
24. Either of the claim 20-22 [origonukureochido] of statement and/or the microphone lower ray of statement is included in claim 23 in 1 sections, the kit for functional deficiency type mutation detection of GmFAD2-1a gene or the GmFAD2-1b gene.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • SAGA UNIVERSITY
  • 発明者(英語)
  • ANAI TOYOAKI
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT RO RS RU SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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