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Method for producing retinal neurocyte from neural stem cell derived from iris tissue

外国特許コード F110005448
整理番号 K05204US
掲載日 2011年9月6日
出願国 アメリカ合衆国
出願番号 55978404
公報番号 20060134280
公報番号 8569056
出願日 平成16年6月11日(2004.6.11)
公報発行日 平成18年6月22日(2006.6.22)
公報発行日 平成25年10月29日(2013.10.29)
国際出願番号 JP2004008222
国際公開番号 WO2004111213
国際出願日 平成16年6月11日(2004.6.11)
国際公開日 平成16年12月23日(2004.12.23)
優先権データ
  • 特願2003-166646 (2003.6.11) JP
  • 2004WO-JP08222 (2004.6.11) WO
発明の名称 (英語) Method for producing retinal neurocyte from neural stem cell derived from iris tissue
発明の概要(英語) (US8569056)
A method for producing retinal nerve cells by inducing differentiation of iris pigmented epithelial cells into the retinal nerve cells.
In a first method, iris pigmented epithelial cells derived from a mammal and embryo retinal stem cells derived from a bird are co-cultured.
In a second method, iris pigmented epithelial cells of a bird or a mammal is isolated, and the iris pigmented epithelial- cells is subjected to adherent culturing.
According to these methods, the retinal nerve cells can be produced by using iris pigmented epithelial cells collected from a patient per se.
Therefore, there is a possibility that highly effective regenerative medical treatment can be realized.
特許請求の範囲(英語) [claim1]
1. A method for producing rhodopsin-positive retinal nerve cells, the method comprising the steps of: isolating iris pigmented epithelial cells from an eyeball;
selectively culturing the iris pigmented epithelial cells isolated from the eyeball by a floated coagulated mass culturing technique; and
performing adherent culturing of the iris pigmented epithelial cells obtained in the selectively culturing step with a serum-free culture medium so as to induce differentiation of the iris pigmented epithelial cells into the rhodopsin-positive retinal nerve cells, the iris pigmented epithelial cells not being subjected to a gene transfer,
the serum-free culture medium containing at least one of a fibroblast growth factor 2, a fibroblast growth factor 9, and a ciliary neurotrophic factor with a concentration in a range of 1 to 100 ng/mL,
the iris pigmented epithelial cells in the serum-free culture medium when the adherent culturing starts having a cell density of 1 * 105 cells/cm2 or less,
the serum-free culture medium being a DMEM/F12 culture medium, a DMEM culture medium, or an EMEM culture medium.
[claim2]
2. The method according to claim 1, wherein the iris pigmented epithelial cells are derived from a bird or a mammal.
[claim3]
3. The method according to claim 1, wherein the fibroblast growth factor 2 and the fibroblast growth factor 9 stop being added after two to five days from the start of the adherent culturing, and the ciliary neurotrophic factor is continuously added from the start of the adherent culturing to the end of the adherent culturing.
[claim4]
4. The method according to claim 1, wherein the iris pigmented epithelial cells are derived from an adult mammal.
  • 発明者/出願人(英語)
  • KOSAKA MITSUKO
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 435/368
  • 435/7.1
  • 435/325
  • 435/377
参考情報 (研究プロジェクト等) PRESTO Time's Arrow and Biosignaling AREA
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