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Method of measuring external stimulus and volume change using stimulus-responsive gel comprising EDANS 新技術説明会

外国特許コード F110005475
整理番号 K05330WO
掲載日 2011年9月6日
出願国 アメリカ合衆国
出願番号 91913306
公報番号 20100063771
公報番号 8999725
出願日 平成18年4月24日(2006.4.24)
公報発行日 平成22年3月11日(2010.3.11)
公報発行日 平成27年4月7日(2015.4.7)
国際出願番号 JP2006308556
国際公開番号 WO2006118077
国際出願日 平成18年4月24日(2006.4.24)
国際公開日 平成18年11月9日(2006.11.9)
優先権データ
  • 2006JP308556 (2006.4.24) WO
  • 特願2005-133431 (2005.4.28) JP
発明の名称 (英語) Method of measuring external stimulus and volume change using stimulus-responsive gel comprising EDANS 新技術説明会
発明の概要(英語) In one embodiment of the present application, a stimulus-responsive gel is disclosed with optical characteristic molecule introduced therein that is capable of easily and securely converting a volume change responding to external stimulus to optical data being easy to handle in the construction of sensor system; an external stimulus measuring apparatus making use of the same; and a method of measuring external stimulus therewith.
A molecule having fluorescent chromophore or a molecule or a molecule capable of absorbing visible light or ultraviolet light is introduced in a stimulus-responsive gel so that the content of the molecule having fluorescent chromophore or the molecule or a molecule capable of absorbing visible light or ultraviolet light falls within such a range that the concentration of the molecule having fluorescent chromophore or a molecule or the molecule capable of absorbing visible light or ultraviolet light in the stimulus-responsive gel with optical characteristic molecule introduced therein is substantially proportional to the fluorescence intensity or absorbance of the visible light or ultraviolet light of the stimulus-responsive gel with optical characteristic molecule introduced therein.
特許請求の範囲(英語) [claim1]
1. A method of measuring an external stimulus and a volume change in a sensor element, the method comprising: (a) creating an analytical curve by plotting logarithms of the volume change against logarithms of fluorescence intensity or absorbance of the visible light or ultraviolet light, by (i) exposing a sensor element to external stimuli of different known ionic concentration, and a liquid, wherein the sensor element comprises a stimulus-responsive gel comprising polyacrylic acid, wherein the stimulus-responsive gel comprises optically active molecules, which are 5-(2'-aminoethyl)aminonaphthalene-1-sulfonate (EDANS), wherein the stimulus responsive gel changes its volume in response to an external stimulus and the sensor element converts into optical data a volume change in the stimulus-responsive gel, and wherein the liquid is absorbed in or discharged out of the sensor element in response to the external stimuli, so as to cause volume changes in the sensor element, (ii) measuring the volume change in the sensor element exposed to the respective external stimuli of different known ionic concentrations, (iii) irradiating light of a particular wavelength on the sensor element exposed to the respective external stimuli of the different known ionic concentrations, and (iv) measuring fluorescence intensity or absorbance of visible light or ultraviolet light therein;
(b) (i) exposing the sensor element to an external stimulus of an unknown ionic concentration in the presence of the liquid, (ii) irradiating the light of the particular wavelength on the sensor element exposed to the external stimuli of the unknown ionic concentration, and (iii) measuring the fluorescence intensity or the absorbance of the visible light or the ultraviolet light in the sensor element; and
(c) determining how large the volume change in the sensor element and the external stimulus of the unknown ionic concentration are, from the measured fluorescence intensity or the absorbance of the visible light or ultraviolet light by using the analytical curve.
[claim2]
2. The method as set forth in claim 1, wherein a concentration of the optically active molecules in the stimulus-responsive gel is in a range between about 0.01% w/w to about 10% w/w relative to the dried weight of the stimulus-responsive gel comprising the optically active molecules.
[claim3]
3. The method as set forth in claim 1, wherein the optically active molecules are chemically bonded to the polyacrylic acid forming the stimulus-responsive gel.
[claim4]
4. The method as set forth in claim 1, wherein the stimulus-responsive gel changes its volume by swelling or shrinking in response to the external stimulus by absorbing or discharging a liquid, wherein the liquid comprises water or an organic solvent.
[claim5]
5. The method as set forth in claim 1, wherein the concentration of optically active molecules in the stimulus-responsive gel is in a range between about 0.1% w/w to about 5% w/w relative to the dried weight of the stimulus-responsive gel comprising the optically active molecules.
[claim6]
6. The method as set forth in claim 1, wherein the logarithms of the volume change in the stimulus-responsive gel and the logarithms of the fluorescence intensity or absorbance of the visible light or ultraviolet light have a linear relationship.
  • 発明者/出願人(英語)
  • MIYATA TAKASHI
  • URAGAMI TADASHI
  • OKAWA KAORI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 436/172
  • 436/56
  • 436/164
  • 436/166
  • 436/169
参考情報 (研究プロジェクト等) PRESTO Conversion and Control by Advanced Chemistry AREA
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