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Nucleic-acid-responsive gel, method for producing same, and use of same 新技術説明会

外国特許コード F110005478
整理番号 K05334WO
掲載日 2011年9月6日
出願国 アメリカ合衆国
出願番号 44885707
公報番号 20100081204
公報番号 8937171
出願日 平成19年8月6日(2007.8.6)
公報発行日 平成22年4月1日(2010.4.1)
公報発行日 平成27年1月20日(2015.1.20)
国際出願番号 JP2007065384
国際公開番号 WO2008084571
国際出願日 平成19年8月6日(2007.8.6)
国際公開日 平成20年7月17日(2008.7.17)
優先権データ
  • 特願2007-005227 (2007.1.12) JP
  • 2007JP065384 (2007.8.6) WO
発明の名称 (英語) Nucleic-acid-responsive gel, method for producing same, and use of same 新技術説明会
発明の概要(英語) The present invention provides a nucleic-acid-responsive gel which allows (i) a larger volumetric change through structural design, (ii) adjustment of its recognition ability to recognize a nucleic acid, (iii) improvement of sensitivity, and (iv) flexible design according to, e.g., a sequence of target DNA.
The nucleic-acid-responsive gel includes a probe formed of two single-stranded nucleic acids which are hybridized with each other.
The probe is fixed within a network structure of a polymer gel.
The two single-stranded nucleic acids are bound reversibly with each other.
特許請求の範囲(英語) [claim1]
1. A nucleic-acid-responsive gel comprising a probe fixed inside a network structure of a polymer gel, wherein the probe comprises 0.1% or more of the dry weight of the gel, and wherein the probe comprises a first single strand of DNA, RNA, or PNA and a second single strand of DNA, RNA, or PNA, wherein the first and the second single strand are substantially complementary to each other;
wherein the first or the second single strand is capable of hybridizing with a target nucleic acid;
wherein the probe forms a cross-linkage in a manner such that each of the first and second single strands of DNA, RNA, or PNA binds at a single point with a polymer compound constituting the network structure of the polymer gel, the single point being the only point where each of the first and second single strands is bound to the polymer compound;
wherein the nucleic-acid-responsive gel swells in response to the target nucleic acid; and wherein the nucleic-acid-responsive gel has a swelling ratio of 1.1 or higher, the swelling ratio being a value obtained by dividing a volume of the gel after a volumetric change after the gel swells by a volume of the gel before the volumetric change.
[claim2]
2. The nucleic-acid-responsive gel as set forth in claim 1, wherein the first and second single strands of DNA, RNA, or PNA have one or more base mismatches relative to each other when the first and the second single strands of DNA, RNA, or PNA are hybridized with each other.
[claim3]
3. The nucleic-acid-responsive gel as set forth in claim 1, wherein: each of the first and the second single strands of DNA, RNA, or PNA has a 5' terminal bound with the polymer compound constituting the network structure of the polymer gel.
[claim4]
4. The nucleic-acid-responsive gel as set forth in claim 1, wherein: each of the first and the second single strands of DNA, RNA, or PNA has a 3' terminal bound with the polymer compound constituting the network structure of the polymer gel.
[claim5]
5. The nucleic-acid-responsive gel as set forth in claim 1, wherein: the first and the second single strands are both DNA, both RNA, or both PNA.
[claim6]
6. The nucleic-acid-responsive gel as set forth in claim 1, wherein: the polymer gel is: obtained by polymerizing a monomer including at least one monomer selected from the group consisting of (meth)acrylamide, (meth)acrylic acid, 2-hydroxyethyl (meth)acrylate, alkyl (meth)acrylate, N,N'-dimethyl (meth)acrylamide, N-isopropyl (meth)acrylamide, vinyl acetate, and alkylamine; or
obtained by reacting the polymer compound selected from the group consisting of poly (meth) acrylamide, poly(meth) acrylic acid, poly-2-hydroxyethyl methacrylate, polyalkyl (meth)acrylate, poly-N,N'dimethyl (meth)acrylamide, poly-N-isopropyl (meth)acrylamide, polyvinyl alcohol, polyalkylamine, cellulose, chitosan, alginic acid, and derivatives thereof with a cross-linking agent so that the polymer gel has the network structure.
[claim7]
7. The nucleic-acid-responsive gel as set forth in claim 1, wherein: the number of bases of the first and the second single strands of DNA, RNA, or PNA is 2 or more, but 10,000 or less.
[claim8]
8. The nucleic-acid-responsive gel as set forth in claim 1, wherein: the nucleic-acid-responsive gel decreases in cross-link density in response to the target nucleic acid that forms a more stable double strand in combination with any one of the first and second single strands of DNA, RNA, or PNA compared to a double strand formed by each of the first and second single strands of DNA, RNA, or PNA hybridized with each other.
[claim9]
9. The nucleic-acid-responsive gel as set forth in claim 1, wherein the nucleic-acid responsive gel is obtained by copolymerizing the probe with a monomer in presence or absence of a cross-linking agent, and further wherein each of the first and the second single strands of DNA, RNA, or PNA has a reactive functional group, and further wherein the monomer includes at least one monomer selected from the group consisting of (meth)acrylamide, (meth)acrylic acid, 2-hydroxyethyl (meth)acrylate, alkyl (meth)acrylate, N,N'-dimethyl (meth)acrylamide, N-isopropyl (meth)acrylamide, vinyl acetate, and allylamine.
[claim10]
10. The nucleic-acid-responsive gel as set forth in claim 1, wherein the nucleic-acid-responsive gel is obtained by a process comprising: (a) binding the probe to the polymer compound, wherein the probe is formed by the first and the second single strands of DNA, RNA, or PNA hybridized with each other, and wherein each of the first and the second single strands has a reactive functional group; and
(b) reacting a mixture comprising the probe and the polymer compound obtained in the step (a) with a cross-linking agent, thereby forming the network structure of the polymer gel, the polymer compound being at least one selected from the group consisting of poly(meth)acrylamide, poly(meth)acrylic acid, poly-2-hydroxyethyl methacrylate, polyalkyl (meth)acrylate, poly-N,N'-dimethyl (meth)acrylamide, poly-N-isopropyl (meth)acrylamide, polyvinyl alcohol, polyalkylamine, cellulose, chitosan, alginic acid, and derivatives thereof.
[claim11]
11. A nucleic acid detection kit comprising the nucleic-acid-responsive gel recited in claim 1.
[claim12]
12. A nucleic acid detection apparatus comprising the nucleic-acid-responsive gel recited in claim 1.
  • 発明者/出願人(英語)
  • MIYATA TAKASHI
  • URAGAMI TADASHI
  • OKAWA KAORI
  • A SCHOOL CORPORATION KANSAI UNIVERSITY
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 536/24.3
  • 204/450
  • 422/68.1
  • 435/6.1
  • 435/6.11
  • 435/91.1
  • 436/94
  • 536/23.1
  • 536/24.33
参考情報 (研究プロジェクト等) PRESTO Conversion and Control by Advanced Chemistry AREA
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