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Method for detecting target nucleic acid with specific base sequence and set of nucleic acids for detection

外国特許コード F110005554
整理番号 N081-31WO
掲載日 2011年9月7日
出願国 アメリカ合衆国
出願番号 08510106
公報番号 20090221429
公報番号 07851159
出願日 平成20年5月16日(2008.5.16)
公報発行日 平成21年9月3日(2009.9.3)
公報発行日 平成22年12月14日(2010.12.14)
国際出願番号 PCT/JP2006/323031
国際公開番号 WO2007/058326
国際出願日 平成18年11月17日(2006.11.17)
国際公開日 平成19年5月24日(2007.5.24)
優先権データ
  • 特願2005-332424 (2005.11.17) JP
発明の名称 (英語) Method for detecting target nucleic acid with specific base sequence and set of nucleic acids for detection
発明の概要(英語) It is intended to provide a method for detecting a target nucleic acid with a specific base sequence existing in a sample mixture with high specificity and sensitivity by utilizing the formation of a hybrid with a complementary strand as a detection principle, and a nucleic acid for the detection. The invention relates to a set of nucleic acids for detecting a target nucleic acid with a specific base sequence comprising a photoligating nucleic acid composed of a nucleic acid (with the proviso that in the nucleic acid, a nucleic acid and a peptide nucleic acid are included) having a group represented by the formula I, II, III, IV or V described in claims at the 5' end or 3' end as a base moiety, and a photoligated nucleic acid having a base with a carbon-carbon double bond at the 3' end or 5' end as a base moiety capable of photoligating to the photoligating nucleic acid, wherein either of the photoligating nucleic acid and the photoligated nucleic acid has a labeling moiety and the other remaining nucleic acid is immobilized on a substrate, and the method for detecting a target nucleic acid with a specific base sequence by using the set of nucleic acids.
  • 発明者/出願人(英語)
  • Fujimoto, Kenzo; Nomi [JP]
  • Yoshimura, Yoshinaga; Ishikawa [JP]
  • Ogasawara, Shinzi; Ishikawa [JP]
  • Japan Science and Technology Agency, Saitama [JP]
国際特許分類(IPC)
米国特許分類/主・副
  • 435/6
  • 536/23.1
  • 536/24.3
  • 536/24.33
  • 536/26.6
参考情報 (研究プロジェクト等) CREST Nano Virtual Lab (Virtual Laboratory in Nanotechnology Areas) Creation of Innovative Technology by Integration of Nanotechnology with Information. Biological, and Environmental Technologies AREA
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