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Linker compound, ligand, and producing method thereof 実績あり

外国特許コード F110005561
整理番号 P028P01US
掲載日 2011年9月8日
出願国 アメリカ合衆国
出願番号 52677503
公報番号 20060030699
公報番号 7320867
出願日 平成15年9月8日(2003.9.8)
公報発行日 平成18年2月9日(2006.2.9)
公報発行日 平成20年1月22日(2008.1.22)
国際出願番号 JP2003011417
国際公開番号 WO2004022583
国際出願日 平成15年9月8日(2003.9.8)
国際公開日 平成16年3月18日(2004.3.18)
優先権データ
  • 特願2002-263412 (2002.9.9) JP
  • 特願2003-190568 (2003.7.2) JP
  • 2003WO-JP11417 (2003.9.8) WO
発明の名称 (英語) Linker compound, ligand, and producing method thereof 実績あり
発明の概要(英語) (US7320867)
A linker compound has a structure represented by general formula (1) below, where n is an integer of 1 to 6, and X has a structure serving as a multi-branched structure moiety including three or four hydrocarbon derivative chains each having an aromatic amino group at an end and a carbon-nitrogen bond in a backbone.
A ligand includes the linker compound and a sugar introduced into the linker compound
特許請求の範囲(英語) [claim1]
1. A linker compound comprising: a structure represented by following general formula (1):
where n is an integer of 1 to 6, andX has a structure represented by the following general formula (2):
where m1, m2 and m3 are independently an integer of 1 to 6.
[claim2]
2. The linker compound according to claim 1, wherein m1, m2, and m3 are all 2 in said general formula (2).
[claim3]
3. A linker compound comprising: a structure represented by following general formula (1):
where n is an integer of 1 to 6, andX has a structure represented by following formula (3):
where m4, m5, m6, m7, p1, and p2 are independently an integer of 1 to 6.
[claim4]
4. The linker compound according to claim 3, wherein m4, m5, m6, and m7 are all 2 and p1 and p2 are both 1 in the general formula (3).
[claim5]
5. A ligand which comprises the aromatic amino group of the linker compound according to claim 1, and a sugar molecule introduced into the aromatic amino group.
[claim6]
6. The ligand according to claim 5, wherein the sugar molecule is at least one kind of sugar selected from the group consisting of a monosaccharide, an oligosaccharide, and a polysaccharide.
[claim7]
7. A ligand of a structure represented by following general formula (4):
wherein m1, m2, m3, and n are independently an integer of 1 to 6.
[claim8]
8. The ligand according to claim 7, wherein m1, m2, and m3 are all 2 and n is 1 in the general formula (4).
[claim9]
9. A ligand comprising a structure represented by following formula (5):
wherein m4, m5, m6, m7, n, p1, and p2 are independently an integer of 1 to 6.
[claim10]
10. The ligand according to claim 9, wherein m4, m5, m6, and m7 are all 2, and n is 1, and p1 and p2 are both 1 in the general formula (5).
[claim11]
11. A method of producing the linker compound of claim 1, comprising: carrying out a condensation reaction between thioctic acid and an amine compound whose aromatic amino group end is protected by a protecting group which is a t-butoxycarbonyl group, a benzyl group, or an allyl carbamate group, the amine compound being represented by the following general formula (19):
where n, m1, m2 and m3 are independently an integer of 1 to 6;
and
deprotecting the protecting group at the aromatic amino group end.
[claim12]
12. A method of producing a ligand, comprising carrying out a reductive amination reaction between the linker compound according to claim 1, and a sugar molecule.
[claim13]
13. A method of immobilizing a ligand containing a sugar molecule on a surface of a support, which comprises, allowing a solution containing the ligand of claim 5 to come into contact with the surface of the support whose surface has a metal, to form a S-metal bond between a S -- S bond contained in the ligand of claim 5 and the metal of the surface of the support, via which S-metal bond, a sugar molecule contained in the ligand of claim 5 is immobilized on the surface of the support.
[claim14]
14. A ligand carrier which comprises the ligand of claim 5 immobilized on a surface of a support whose surface has a metal.
[claim15]
15. The ligand carrier according to claim 14, wherein the support is a sensor chip for a surface plasmon resonance measurement.
[claim16]
16. The ligand carrier according to claim 14, wherein the support is a column for affinity chromatography.
[claim17]
17. A method of immobilizing a ligand containing a sugar molecule on a surface of a support, which comprises, allowing a solution containing the ligand of claim 7 to come into contact with the surface of the support whose surface has a metal, to form a S-metal bond between a S -- S bond contained in the ligand of claim 7 and the metal of the surface of the support, via which S-metal bond, a sugar molecule contained in the ligand of claim 7 is immobilized on the surface of the support.
[claim18]
18. A ligand carrier which comprises the ligand of claim 7 immobilized on a surface of a support whose surface has a metal.
[claim19]
19. The ligand carrier according to claim 18, wherein the support is a sensor chip for a surface plasmon resonance measurement.
[claim20]
20. The ligand carrier according to claim 18, wherein the support is a column for affinity chromatography.
[claim21]
21. A method of immobilizing a ligand containing a sugar molecule on a surface of a support, which comprises, allowing a solution containing the ligand of claim 9 to come into contact with the surface of the support whose surface has a metal, to form a S-metal bond between a S -- S bond contained in the ligand of claim 9 and the metal of the surface of the support, via which S-metal bond, a sugar molecule contained in the ligand of claim 9 is immobillized on the surface of the support.
[claim22]
22. A ligand carrier which comprises the ligand of claim 9 immobilized on a surface of a support whose surface has a metal.
[claim23]
23. The ligand carrier according to claim 22, wherein the support is a sensor chip for a surface plasmon resonance measurement.
[claim24]
24. The ligand carrier according to claim 22, wherein the support is a column for affinity chromatography.
[claim25]
25. A method of producing the linker compound of claim 3, comprising: carrying out a condensation reaction between thioctic acid and an amine compound whose aromatic amino group end is protected by a protecting group which is a t-butoxycarbonyl group, a benzyl group, or an allyl carbamate group, the amine compound being represented by the following general formula (20):
where n, m4, m5, m6, m7, p1, and p2, are independently an integer of 1 to 6;
and deprotecting the protecting group at the aromatic amino group end.
  • 発明者/出願人(英語)
  • SUDA YASUO
  • ARANO AKIO
  • KUSUMOTO SHOICHI
  • SOBEL MICHAEL
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • KAGOSHIMA UNIVERSITY
国際特許分類(IPC)
米国特許分類/主・副
  • 435/7.1
  • 435/7.92
  • 435/137
  • 435/287.2
  • 435/961
  • 435/970
  • 436/544
  • 530/402
  • 530/404
  • 530/411
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