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Carbohydrate-ligand conjugates and their application for the analysis of carbohydrate-protein interaction 実績あり

外国特許コード F110005564
整理番号 P028P08WO
掲載日 2011年9月8日
出願国 アメリカ合衆国
出願番号 59004505
公報番号 20070287195
公報番号 8765384
出願日 平成17年2月18日(2005.2.18)
公報発行日 平成19年12月13日(2007.12.13)
公報発行日 平成26年7月1日(2014.7.1)
国際出願番号 JP2005003220
国際公開番号 WO2005077965
国際出願日 平成17年2月18日(2005.2.18)
国際公開日 平成17年8月25日(2005.8.25)
優先権データ
  • 特願2004-041994 (2004.2.18) JP
  • 2005JP003220 (2005.2.18) WO
発明の名称 (英語) Carbohydrate-ligand conjugates and their application for the analysis of carbohydrate-protein interaction 実績あり
発明の概要(英語) A novel ligand conjugate which is effectively utilizable for analyzing a function of a protein; a ligand-supporting object; and a method of analyzing a protein.
The ligand conjugate has a structure which comprises: a linker compound having a structure represented by the following General Formula (1): (wherein n and p each is an integer of 0 to 6) in which X is a structure comprising one, two, or three hydrocarbon derivative chains which have an aromatic amino group at the end and may have a carbon-nitrogen bond in the main chain, Y is a hydro-carbon structure containing one or more sulfur atoms, and Z is a straight-chain structure comprising a carbon-carbon bond or carbon-oxygen bond; and a sugar which has a reducing end and is bonded to the linker compound through the aromatic amino group.
特許請求の範囲(英語) [claim1]
1. A ligand conjugate comprising a linker compound and a sugar chain, the linker compound having a structure represented by General Formula (1):
where p and q are independently integers of not less than 1 but not more than 6, in which
X is a structure represented by formula 3:
wherein m4 and m5 are each independently integers of not less than 1 but not more than 6, and R' is a hydrogen (H) or R,
Y is a hydrocarbon structure having an S -- S bond or an S -- H group,
Z is a straight-chain structure comprising a carbon-carbon bond or carbon-oxygen bond, and
R comprises a substituent derived from the sugar chain selected from the group consisting of:
[claim2]
2. The ligand conjugate of claim 1, wherein: Z has a structure of Formula (5) or (6):
where n1 and n2 are independently integers of not less than 1 but not more than 6.
[claim3]
3. The ligand conjugate as set forth in claim 1 having a structure represented by General Formula (108):
where n1 is an integer of not less than 1 but not more than 6.
[claim4]
4. A ligand carrier in which the ligand conjugate as set forth in any one of claims 1, 2 or 3 is immobilized on a support having a metal on a surface thereof.
[claim5]
5. A method for analyzing protein, comprising: allowing the ligand conjugate as set forth in any one of claim 1, 2, or 3 to come in contact with a support having a metal on a surface thereof so as to prepare a ligand carrier in which the ligand conjugate is immobilized on the support;
analyzing intermolecular interaction by surface plasmon resonance (SPR) after allowing the ligand carrier to come in contact with a protein solution; and
performing mass spectroscopy after the analysis of the intermolecular interaction, so as to identify a protein bound on the ligand carrier.
[claim6]
6. The ligand conjugate as set forth in claim 1, wherein m4 and m5 are each 2.
[claim7]
7. A method for analyzing protein, comprising: allowing the ligand carrier of claim 4 to come in contact with a protein solution, and analyzing intermolecular interaction by SPR measurement.
  • 発明者/出願人(英語)
  • SUDA YASUO
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • KAGOSHIMA UNIVERSITY
国際特許分類(IPC)
米国特許分類/主・副
  • 435/7.1
  • 435/7.92
  • 436/525
  • 436/544
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