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Photoresponsive nucleic acid manufacturing method

外国特許コード F110005604
整理番号 RX02P34WO
掲載日 2011年9月9日
出願国 アメリカ合衆国
出願番号 86769409
公報番号 20110040083
公報番号 8642754
出願日 平成21年2月13日(2009.2.13)
公報発行日 平成23年2月17日(2011.2.17)
公報発行日 平成26年2月4日(2014.2.4)
国際出願番号 JP2009000562
国際公開番号 WO2009101810
国際出願日 平成21年2月13日(2009.2.13)
国際公開日 平成21年8月20日(2009.8.20)
優先権データ
  • 特願2008-033713 (2008.2.14) JP
  • 2009JP000562 (2009.2.13) WO
発明の名称 (英語) Photoresponsive nucleic acid manufacturing method
発明の概要(英語) The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology.
The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent.
特許請求の範囲(英語) [claim1]
1. A method of manufacturing a nucleic acid having a group represented by the following Formula XV:
in the Formula XV, X represents O, S or NH, R4 represents a hydrogen atom, a C1 to C6 alkyl group, a C1 to C6 alkoxy group, a cyano group or a C1 to C6 acyl group, and
R3 represents a monovalent group of a substituted or unsubstituted aromatic compound, or a cyano group,
wherein the method comprises the following step (b):
(b) reacting a nucleic acid having a base sequence comprising a nucleotide base moiety represented by Formula VI with a compound represented by Formula VII in the presence of a palladium complex catalyst, a basic substance and a solvent by microwave heating:
in the Formula VI, X represents O, S or NH,
R4 represents a hydrogen atom, a C1 to C6 alkyl group, a C1 to C6 alkoxy group, a cyano group or a C1 to C6 acyl group, and
A2 represents a halogen atom;
R3 -- B(OH)2 (VII)
in the Formula VII, R3 represents a monovalent group of a substituted or unsubstituted aromatic compound, or a cyano group.
[claim2]
2. The method according to claim 1, wherein the step (b) is carried out by heating by microwaves in the presence of a palladium complex catalyst, a basic substance, a solvent, and an aqueous solution of carboxylate.
[claim3]
3. The method according to claim 1, wherein the basic substance is trialkylamine having a C1 to C6 alkyl group.
[claim4]
4. The method according to claim 1, wherein the step (b) is carried out by heating by microwaves in the presence of a palladium complex catalyst, a solvent, and an aqueous solution of carboxylate.
[claim5]
5. The method according to claim 2, wherein the aqueous solution of carboxylate is an aqueous solution of an alkali metal salt of C1 to C3 carboxylic acid.
[claim6]
6. The method according to claim 2, wherein the aqueous solution of carboxylate is a buffer solution having a pH range of 4.5 to 6.0.
[claim7]
7. The method according to claim 1, wherein the heating by microwaves is carried out in the temperature range of 70 to 140 deg. C.
[claim8]
8. The method according to claim 1, wherein the heating by microwaves is carried out in the time range of 1 to 30 minutes.
[claim9]
9. The method according to claim 1, wherein the solvent is an aprotic polar solvent.
  • 発明者/出願人(英語)
  • FUJIMOTO KENZO
  • OGINO MASAYUKI
  • YOSHIMURA YOSHINAGA
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 536/25.32
  • 536/24.3
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