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Blood analysis device and blood analysis method

外国特許コード F110005651
整理番号 V123P002US
掲載日 2011年9月9日
出願国 アメリカ合衆国
出願番号 54644704
公報番号 20060078873
公報番号 7582259
出願日 平成16年2月18日(2004.2.18)
公報発行日 平成18年4月13日(2006.4.13)
公報発行日 平成21年9月1日(2009.9.1)
国際出願番号 JP2004001802
国際公開番号 WO2004074846
国際出願日 平成16年2月18日(2004.2.18)
国際公開日 平成16年9月2日(2004.9.2)
優先権データ
  • 特願2003-040481 (2003.2.19) JP
  • 2004WO-JP01802 (2004.2.18) WO
発明の名称 (英語) Blood analysis device and blood analysis method
発明の概要(英語) (US7582259)
A blood analysis device for centrifugally separating plasma in a channel, wherein conveyance of blood, plasma and calibration liquid is effected within the device without using a pump or the like.
The calibrator solution is reliably discharged from a sensor portion so as to make high precision analysis possible.
A sensor section is provided in a plasma separating section and disposed on the side associated with a first centrifugal pressing direction as seen from a blood reservoir and a calibrator solution reservoir, while a calibrator solution waste reservoir is disposed in a second centrifugal pressing direction as seen from the plasma separating section (sensor section).
The calibrator solution is conveyed to the sensor section by centrifugal operation in the first centrifugal direction.
After sensor calibration, the calibrator solution can be reliably discharged from the sensor section by effecting centrifuging in the second centrifugal direction.
After the calibrator solution discharge, centrifuging is effected again in the first centrifugal direction, thereby conveying the blood in the blood reservoir to the sensor section and effecting separation of blood cells and plasma.
In the case of providing a plurality of sensors, a blood introducing channel from the blood reservoir is branched downwardly of a sensor groove for communication, with the blood cells being fractionated in the branch section.
The individual sensors can be isolated from each other by blood cell fraction, making higher precision analysis possible.
特許請求の範囲(英語) [claim1]
1. A blood analysis device which separates a plasma from a whole blood sample by centrifugation and analyzes a component to be analyzed in blood liquid components, comprising: (a) a substrate comprising a sensor for analyzing the component to be analyzed in the blood liquid components;
(b) a plasma separating section formed in said substrate, and having a sensor groove for housing the sensor, the plasma being separated in the sensor groove when a centrifugal force is applied to the substrate in a first centrifugal direction;
(c) a blood introducing channel for communicating with said plasma separating section so that a blood sample is introduced into said plasma separating section when the centrifugal force is applied to the substrate in the first centrifugal direction;
(d) a calibrator solution introducing channel for communicating with said plasma separating section so that a calibrator solution is introduced into said plasma separating section, when the centrifugal force is applied to the substrate in the first centrifugal direction;
(e) a calibrator solution waste reservoir for communicating with said plasma separating section and allowing the calibrator solution in said plasma separating section to move therein, when the centrifugal force is applied to the substrate in a second centrifugal direction; and
(f) a calibrator solution discharge channel which allows said plasma separating section to communicate with said calibrator solution waste reservoir, and discharges the calibrator solution in said plasma separating section to said calibrator solution waste reservoir, when the centrifugal force is applied to the substrate in the second centrifugal direction;
wherein said sensor groove is positioned to discharge the calibrator solution from the sensor groove by action of the centrifugal force in the second centrifugal direction; and
wherein said calibrator solution waste reservoir is positioned and said calibrator solution discharge channel is disposed so that the calibrator solution contained in the calibrator solution waste reservoir does not flow back into said plasma separating section when the centrifugal force is applied to the substrate in the first centrifugal direction.
[claim2]
2. The blood analysis device according to claim 1, wherein said plasma separating section comprises a plurality of sensor grooves which house a plurality of sensors for analyzing different components to be analyzed, and said blood introducing channel is branched to communicate with each of the plurality of sensor grooves on the side associated with the first centrifugal pressurizing direction of the plasma separating section.
[claim3]
3. The blood analysis device according to claim 2, wherein a portion of the blood introducing channel positioned on the side associated with the first centrifugal force pressurizing direction from said plasma separating section has a capacity to receive a blood cell fraction component in blood in a case where the centrifugal force is applied to the substrate in the first centrifugal direction, and the plasma brought into contact with one of said sensors is isolated from that brought into contact with the other sensor by a blood cell fraction.
[claim4]
4. The blood analysis device according to claim 1, wherein the sensor is an electrochemical sensor.
[claim5]
5. The blood analysis device according to claim 1, wherein a blood collecting needle is attachable to a blood intake port of said blood introducing channel.
[claim6]
6. The blood analysis device according to claim 1, wherein said blood introducing channel is subjected to a hydrophilic treatment.
[claim7]
7. The blood analysis device according to claim 1, wherein said calibrator solution introducing channel is subjected to a hydrophilic treatment.
[claim8]
8. The blood analysis device according to claim 1, further comprising a blood reservoir disposed midway in said blood introducing channel.
[claim9]
9. The blood analysis device according to claim 8, wherein said blood reservoir and the blood introducing channel on an upstream side from the blood reservoir are subjected to a hydrophilic treatment.
[claim10]
10. The blood analysis device according to claim 1, wherein a calibrator solution reservoir is disposed midway in said calibrator solution introducing channel.
[claim11]
11. The blood analysis device according to claim 10, wherein said calibrator solution reservoir, and the calibrator solution introducing channel on an upstream side from the calibrator solution reservoir are subjected to a hydrophilic treatment.
[claim12]
12. The blood analysis device according to claim 1, wherein said first and second centrifugal directions cross each other substantially at right angles.
[claim13]
13. A blood analysis method comprising the steps of: (a) providing a blood analysis device comprising a substrate provided with a sensor; a plasma separating section disposed in the substrate, having a sensor groove which houses the sensor, and separating a plasma in the sensor groove; a blood introducing channel which introduces a blood sample into the plasma separating section; a calibrator solution introducing channel which introduces a calibrator solution into the plasma separating section; a calibrator solution waste reservoir; and a calibrator solution discharge channel which connects the plasma separating section to the calibrator solution waste reservoir, and discharges the calibrator solution in the plasma separating section to the calibrator solution waste reservoir;
(b) supplying the calibrator solution to said calibrator solution introducing channel;
(c) applying a centrifugal force to the substrate in a first centrifugal direction in such a manner that said plasma separating section is disposed in a centrifugal force pressurizing direction, so as to introduce the calibrator solution in the calibrator solution introducing channel into said sensor groove of the plasma separating section;
(d) calibrating said sensor;
(e) rotating said substrate with positioning the substrate in a second centrifugal direction to centrifuge the substrate in such a manner that said calibrator solution reservoir is disposed in the centrifugal force pressurizing direction, and discharging the calibrator solution in the sensor groove to the calibrator solution waste reservoir;
(f) introducing a blood sample into said blood introducing channel;
(g) applying the centrifugal force to the substrate in the first centrifugal direction in such a manner as to dispose the plasma separating section in the centrifugal force pressurizing direction, whereby the blood sample is transferred to the plasma separating section with allowing the plasma separating section to separate blood cells from the plasma, so as to introduce the separated plasma into the sensor groove; and
(h) analyzing a liquid component of the plasma in the sensor groove by the sensor.
[claim14]
14. The blood analysis method according to claim 13, wherein said plasma separating section comprises a plurality of sensor grooves, and said blood introducing channel is branched in a first centrifugal direction on the side opposed to each sensor groove to communicate with each sensor groove; and a blood cell fraction in the blood sample is precipitated in a branched portion of said blood introducing channel on the far side associated with the first centrifugal direction by centrifugation in the first direction in said step (g), and the plasma separated as a centrifugal supernatant is positioned in each sensor groove.
[claim15]
15. The blood analysis method according to claim 14, wherein in said steps (g), (h), the plasma introduced into each sensor groove is isolated from the plasma in the other sensor groove by the blood cell fraction which exists in the blood introducing channel branched portion.
[claim16]
16. The blood analysis method according to claim 13, wherein a blood reservoir is provided midway in said blood introducing channel, and the blood reservoir and the blood introducing channel on an upstream side of the blood reservoir are subjected to a hydrophilic treatment; and in said step (f), the blood sample is introduced into the blood reservoir by a capillary function.
[claim17]
17. The blood analysis method according to claim 13, wherein said first and second centrifugal directions cross each other substantially at right angles.
[claim18]
18. The blood analysis device according to claim 2, wherein the sensor is an electrochemical sensor.
[claim19]
19. The blood analysis device according to claim 3, wherein the sensor is an electrochemical sensor.
  • 発明者/出願人(英語)
  • OGAWA HIROKI
  • HORIIKE YASUHIRO
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 422/72
  • 435/4
  • 435/287.1
  • 436/66
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