TOP > 外国特許検索 > Method of measuring interaction between biomaterial and sugar chain, method of evaluating biomaterial in sugar chain selectivity, method of screening biomaterial, method of patterning biomaterials, and kits for performing these methods

Method of measuring interaction between biomaterial and sugar chain, method of evaluating biomaterial in sugar chain selectivity, method of screening biomaterial, method of patterning biomaterials, and kits for performing these methods

外国特許コード F110005672
整理番号 V350P002US
掲載日 2011年9月9日
出願国 アメリカ合衆国
出願番号 81869507
公報番号 20080145838
公報番号 9239329
出願日 平成19年6月15日(2007.6.15)
公報発行日 平成20年6月19日(2008.6.19)
公報発行日 平成28年1月19日(2016.1.19)
優先権データ
  • 特願2006-340554 (2006.12.18) JP
発明の名称 (英語) Method of measuring interaction between biomaterial and sugar chain, method of evaluating biomaterial in sugar chain selectivity, method of screening biomaterial, method of patterning biomaterials, and kits for performing these methods
発明の概要(英語) This invention provides a method of screening or patterning a biomaterial in terms of their specificities to sugar chains by performing real-time and comprehensive measurement of an interaction between sugar chains and the biomaterial concurrently with a very small amount of the biomaterial without labeling.
It is a method of measuring an interaction between a biomaterial and a sugar chain(s), the method including: bringing a solution containing the biomaterial in contact with a ligand carrier, the ligand carrier including a support whose surface includes a metal, and a ligand conjugate(s) immobilized independently on the surface, the ligand carrier carrying the ligand conjugate(s) in such a manner that 1 to 500 kinds of the ligand conjugate(s) is immobilized per cm2, and each ligand conjugate having a structure in which a sugar chain is bonded with a linker compound having a sulfur atom, and the biomaterial being at least one selected from the groups of proteins, viruses, cells, microorganisms, liposome, and micelles.
従来技術、競合技術の概要(英語) BACKGROUND OF THE INVENTION
Sugar chains are compounds in which sugars are bonded via glycoside bonding.
There are infinite combinations of sugars considering kinds and sequences of sugars constituting the sugar chains.
Therefore, there are a great variety of the sugar chains.
It is known that sugar chains interact with various biomaterial such as viruses, cells, microorganisms, proteins, etc.
Thus, the interactions of the sugar chains with the biomaterials largely influence physiological conditions of living organisms.
For example, it is known that infections of influenza virus, AIDS virus, and hepatitis B virus, which causes severe diseases in infected people, are initiated by interaction of these viruses with sugar chains in cell membranes of human cells.
Different viruses interact with different sugar chains.
This accounts for differences of virus infections in their infection routs and symptoms that they cause in humans.
For example, as disclosed in Virology, 1997, 227, 493-499, it is understood that isolated influenza virus strain interact with different sugar chains that are different in constituent sugars and sugar sequences.
It is known that human-infectious influenza viruses generally interact with N-acetyl neuraminic acid alpha 2,6-galactose (Neu5Ac alpha 2,6 Gal) more strongly than N-acetyl neuraminic acid alpha 2,3-galactose (Neu5Ac alpha 2,3 Gal).
Therefore, comprehensive measurement on interactions of a virus with sugar chains is weighed heavily in developing strategies for prevention and remedy against the infection of the virus.

特許請求の範囲(英語) [claim1]
1. A method of classifying influenza virus having H3N2 protein by strains, the method comprising: contacting a solution comprising the influenza virus having H3N2 protein with a ligand carrier, wherein the ligand carrier comprises a support and a plurality of ligand conjugates,
wherein the support comprises a metal on a surface of the support, and each ligand conjugate of the plurality of ligand conjugates comprises a sugar chain bonded to a linker compound comprising a sulfur atom,
wherein the plurality of ligand conjugates are bonded to the surface via metal-sulfur bonding due to the sulfur atom in the linker compound, and
wherein there are 2 to 500 different kinds of the ligand conjugates, each kind of the ligand conjugate having a different sugar chain, immobilized independently on the ligand carrier per cm2, such that the different kinds of the ligand conjugates are bonded to the surface in non-overlapping positions, and wherein the different kinds of ligand conjugates have differing binding specificity to different H3N2 virus strains due to the different sugar chains in the different kinds of ligand conjugates;
measuring interactions between the influenza virus having H3N2 protein and two or more sugar chains of the plurality of ligand conjugates; and
classifying the influenza virus having H3N2 protein by strains, based on differential binding of the influenza virus having H3N2 protein to the different kinds of ligand conjugates, wherein the classifying further comprises determining where the influenza virus having H3N2 protein interacts with the ligand carrier relative to the positions of the different kinds of ligand conjugates of the plurality of ligand conjugates.
[claim2]
2. The method as set forth in claim 1, wherein the structure of the linker compound is represented by:
where X is a structure that includes one hydrocarbon chain which has a terminal aromatic amino group with a carbon-nitrogen bond in its main chain, and Y is a hydrocarbon structure having the sulfur atom, and n is not less than 0 but not more than 6.
[claim3]
3. The method as set forth in claim 1, wherein the sugar chains are at least two selected from the group consisting of: Galbeta 1-4Glc, Galbeta 1-4GlcNAcbeta 1-6Glc, Neu5Acalpha 2-3Galbeta 1-4Glc, Neu5Acalpha 2-6Galbeta 1-4Glc, Neu5Acalpha 2-3Galbeta 1-3GlcNAcbeta 1-6Glc, Neu5Acalpha 2-6Galbeta 1-3GlcNAcbeta 1-6Glc, Neu5Acalpha 2-3Galbeta 1-4GlcNAcbeta 1-6Glc, Neu5Acalpha 2-6Galbeta 1-4GlcNAcbeta 1-6Glc, GlcAbeta 1-3GalNAc4Sbeta 1-6Glc, GlcAbeta 1-3GalNAc6Sbeta 1-6Glc, GlcA2Sbeta 1-3GalNAc6Sbeta 1-6Glc, GlcAbeta 1-3GalNAc4S6Sbeta 1-6Glc, GlcNS6Salpha 1-4IdoA2Salpha 1-6Glc, GlcNSalpha 1-4IdoA2Salpha 1-6Glc, GlcNS6Salpha 1-4GlcA2Sbeta 1-6Glc, and GlcNSalpha 1-4GlcAbeta 1-6Glc.
[claim4]
4. A kit for performing a method of classifying influenza virus having H3N2 protein by strains, the kit comprising: a solution comprising the influenza virus having H3N2 protein; and
a ligand carrier, wherein the ligand carrier comprises a support and a plurality of ligand conjugates, wherein the support comprises a metal on a surface of the support,
and each ligand conjugate of the plurality of ligand conjugates comprises a sugar chain bonded to a linker compound comprising a sulfur atom,
wherein the plurality of ligand conjugates are bonded to the surface via metal-sulfur bonding due to the sulfur atom in the linker compound and wherein there are 2 to 500 different kinds of the ligand conjugates, each kind of the ligand conjugate having a different sugar chain, immobilized independently on the ligand carrier per cm2, such that the different kinds of ligand conjugates are bonded to the surface in non-overlapping positions, and
wherein the different kinds of ligand conjugates have differing binding specificity to the different H3N2 virus strains due to the different sugar chains in the different kinds of ligand conjugates, and
wherein the kit is configured to classify influenza virus having H3N2 protein by strain based on differential binding of the influenza virus to the different kinds of ligand conjugates, wherein the classification comprises determining where the influenza virus interacts with the ligand carrier relative to the positions of the different kinds of ligand conjugates of the plurality of ligand conjugates.
[claim5]
5. The kit as set forth in claim 4, wherein the sugar chains are at least two selected from the group consisting of: Galbeta 1-4Glc, Galbeta 1-4GlcNAcbeta 1-6Glc, Neu5Acalpha 2-3Galbeta 1-4Glc, Neu5Acalpha 2-6Galbeta 1-4Glc, Neu5Acalpha 2-3Galbeta 1-3GlcNAcbeta 1-6Glc, Neu5Acalpha 2-6Galbeta 1-3GlcNAcbeta 1-6Glc, Neu5Acalpha 2-3Galbeta 1-4GlcNAcbeta 1-6Glc, Neu5Acalpha 2-6Galbeta 1-4GlcNAcbeta 1-6Glc, GlcAbeta 1-3GalNAc4Sbeta 1-6Glc, GlcAbeta 1-3GalNAc6Sbeta 1-6Glc, GlcA2Sbeta 1-3GalNAc6Sbeta 1-6Glc, GlcAbeta 1-3GalNAc4S6Sbeta 1-6Glc, GlcNS6Salpha 1-4IdoA2Salpha 1-6Glc, GlcNSalpha 1-4IdoA2Salpha 1-6Glc, GlcNS6Salpha 1-4GlcA2Sbeta 1-6Glc, and GlcNSalpha 1-4GlcAbeta 1-6Glc.
[claim6]
6. The method of claim 1, wherein in the measuring the interactions between the influenza virus having H3N2 protein and the two or more sugar chains of the plurality of ligand conjugates, a strength of the interactions between the influenza virus having H3N2 protein and the two or more sugar chains of the plurality of ligand conjugates is measured.
[claim7]
7. The method of claim 1, wherein in the measuring the interactions between the influenza virus having H3N2 protein and the two or more sugar chains of the plurality of ligand conjugates, the interactions between the influenza virus having H3N2 protein and the two or more sugar chains of the plurality of ligand conjugates are measured concurrently.
  • 発明者/出願人(英語)
  • SUDA YASUO
  • NISHIMURA TOMOAKI
  • KISHIMOTO YUKO
  • YAMASHITA SAKIKO
  • TSURUTA SACHIKO
  • WAKAO MASAHIRO
  • OKUNO TOSHIOMI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 2016/01/01
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