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Method and kit for detecting biological signal of three-dimensional cell culture material

外国特許コード F110005812
整理番号 K02807WO
掲載日 2011年10月19日
出願国 アメリカ合衆国
出願番号 200913128360
公報番号 20110217726
公報番号 8828679
出願日 平成21年11月10日(2009.11.10)
公報発行日 平成23年9月8日(2011.9.8)
公報発行日 平成26年9月9日(2014.9.9)
国際出願番号 JP2009069124
国際公開番号 WO2010055829
国際出願日 平成21年11月10日(2009.11.10)
国際公開日 平成22年5月20日(2010.5.20)
優先権データ
  • 特願2008-289011 (2008.11.11) JP
  • 2009JP069124 (2009.11.10) WO
発明の名称 (英語) Method and kit for detecting biological signal of three-dimensional cell culture material
発明の概要(英語) Disclosed is a method for detecting a biological signal of a three-dimensional cell culture construct.
The method for detecting a biological signal of a three-dimensional cell culture construct includes: providing a three-dimensional cell culture construct that contains at least two cell layers laminated to each other and a sensor particle capable of detecting a biological signal; and observing the sensor particle optically.
Preferably, the three-dimensional cell culture construct contains an extracellular matrix including a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence, or a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged.
特許請求の範囲(英語) [claim1]
1. A method for detecting a biological signal of a construct that is produced by a layer-by-layer (LBL) method, comprising: providing a construct that is produced by an LBL method and contains at least two cell layers, an extracellular matrix, and a sensor particle capable of detecting a biological signal; and
observing the sensor particle optically,
wherein the construct is provided so that the extracellular matrix is formed between the cell layers, thereby laminating the cell layers to one another, and
wherein the construct is provided so that the sensor particle is placed in the extracellular matrix between the cell layers.
[claim2]
2. The method according to claim 1, wherein the sensor particle contains a substance having a sensing function, a carrier for supporting the substance having a sensing function, and a biocompatible basic polymer layer and a biocompatible acidic polymer layer that are laminated alternately on a surface of the carrier, and
the carrier is a porous particle.
[claim3]
3. The method according to claim 1, wherein the optical observation of the sensor particle includes visualization and/or numeric conversion of the biological signal.
[claim4]
4. The method according to claim 1, wherein the extracellular matrix includes a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence, or a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged.
[claim5]
5. The method according to claim 1, wherein the construct is capable of being produced by a process, comprising: forming a cell layer by introducing a cell-containing solution;
forming an extracellular matrix by introducing a first solution and a second solution alternately;
forming the extracellular matrix and the cell layer alternately to laminate the cell layers; and
placing the sensor particle capable of detecting a biological signal between cell layers,
wherein a combination of an ingredient of the first solution and an ingredient of the second solution is a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence, or a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged.
[claim6]
6. The method according to claim 1, wherein the sensor particle contains a substance having a sensing function, a carrier for supporting the substance having a sensing function, and a basic polymer layer and an acidic polymer layer that are laminated alternately on a surface of the carrier.
[claim7]
7. A method for evaluating a construct that is produced by a layer-by-layer (LBL) method, comprising: detecting a biological signal using the method according claim 1; and
analyzing a cellular activity based on a detection result of the biological signal.
[claim8]
8. A method for evaluating a test substance with respect to an organism, comprising: bringing a construct that is produced by a layer-by-layer (LBL) method into contact with a test substance selected from the group consisting of compounds, medical compositions, cosmetics and foods; and
detecting a biological signal of the construct using the method according to claim 1.
  • 発明者/出願人(英語)
  • MATSUSAKI MICHIYA
  • AKASHI MITSURU
  • OKANO KAZUNORI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 435/29
参考情報 (研究プロジェクト等) PRESTO Structures and control of interfaces AREA
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