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METHOD FOR DETECTING MASS CHANGE IN MASS SPECTROMETRY METHOD AND METHOD FOR QUANTIFYING ABSOLUTE AMOUNT OF STABLE ISOTOPE-LABELED PROTEIN 実績あり

外国特許コード F120006304
整理番号 T101105
掲載日 2012年3月15日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2012JP000227
国際公開番号 WO 2012/111249
国際出願日 平成24年1月17日(2012.1.17)
国際公開日 平成24年8月23日(2012.8.23)
優先権データ
  • 特願2011-029205 (2011.2.14) JP
発明の名称 (英語) METHOD FOR DETECTING MASS CHANGE IN MASS SPECTROMETRY METHOD AND METHOD FOR QUANTIFYING ABSOLUTE AMOUNT OF STABLE ISOTOPE-LABELED PROTEIN 実績あり
発明の概要(英語)

Conventional highly sensitive protein quantification methods using a spectrometer had the issue of sample loss and non-fragmentation rates during the process of fragmentation of the target protein not being corrected by internal standard peptide fragments. In addition, identification and/or quantification of posttranslational modification etc., of proteins using a spectrometer was extremely difficult. The present invention addresses the problem of developing a method for high-precision quantification of proteins using a spectrometer and identification and/or quantification of posttranslational modifications or genetic mutations in proteins. The present inventors found that a plurality of target protein-derived peptide fragments can be detected at one time using the spectrometer and proteins can be quantified with high sensitivity and high precision, by using a stable isotope-labeled, internal standard protein. The present inventors also found that the posttranslational modifications and genetic mutations of each peptide fragment could be identified and/or quantified using the average quantification value for the detected target protein-derived peptide fragments.

  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • SCHOOL CORPORATION, AZABU VETERINARY MEDICINE EDUCATIONAL INSTITUTION,
  • KAMIIE, JUNICHI,
  • KAWAKAMI, HIROTAKA,
  • SAKAUE, MOTOHARU,
  • SHIROTA, KINJI
  • 発明者(英語)
  • KAMIIE, JUNICHI,
  • KAWAKAMI, HIROTAKA,
  • SAKAUE, MOTOHARU,
  • SHIROTA, KINJI
国際特許分類(IPC)
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