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Microscope and a fluorescent observation method using the same

外国特許コード F120006331
整理番号 B115-02WO
掲載日 2012年3月22日
出願国 アメリカ合衆国
出願番号 200913061705
公報番号 20110242308
公報番号 9019360
出願日 平成21年6月10日(2009.6.10)
公報発行日 平成23年10月6日(2011.10.6)
公報発行日 平成27年4月28日(2015.4.28)
国際出願番号 JP2009060644
国際公開番号 WO2010029799
国際出願日 平成21年6月10日(2009.6.10)
国際公開日 平成22年3月18日(2010.3.18)
優先権データ
  • 特願2008-235795 (2008.9.13) JP
  • 2009JP060644 (2009.6.10) WO
発明の名称 (英語) Microscope and a fluorescent observation method using the same
発明の概要(英語) A microscope capable of controlling the position and fluorescent recording of an object under observation such as cells is provided with the fluorescent observation method using the microscope.
The microscope 1 comprises: a stage 3 on which the object under observation 2 is placed; an illumination light source 4 for the object under observation 2; an excitation light source 5 for exciting fluorescent light F to the object under observation 2; an image information detecting part 16 for detecting the image information formed with the light T generated at the object under observation 2; a fluorescent image information detecting part 17 for detecting the fluorescent image information formed with fluorescent light F; and a control part 20, which determines the fluorescent observation area of the object under observation 2 based on the dynamic model of the object under observation 2 and its image information entered from the image information detecting part 16, and then obtains the image information of the object under observation 2 entered from the image information detecting part 16 and the fluorescent image information entered from the fluorescent image information detecting part 17 at specified interval within the fluorescent observation area.
特許請求の範囲(英語) [claim1]
1. A microscope for analyzing a biological tissue, comprising: a stage for placing an object under observation that is the biological tissue;
a first light source with an optical system for radiating illumination light to the object under observation;
a second light source with an optical system for radiating excitation light for exciting fluorescent light to the object under observation;
an image detecting part for detecting an image of the object under observation formed by the illumination light;
a fluorescent image detecting part for detecting a fluorescent image of the biological tissue formed by the fluorescent light, said stage, the fluorescent image detection part, and the second light source being configured to capture a fluorescent image of a horizontal cross section of the biological tissue and a vicinity thereof at multiple levels along a height of the biological tissue; and
a control part that determines a fluorescent observation portion of the biological tissue from which said fluorescent image of said horizontal cross section is taken at the multiple vertical levels,
wherein the control part determines the fluorescent observation portion by: determining a center of the fluorescent observation portion along a vertical direction using at least either one of the image of the biological tissue detected by the image detecting part or a dynamic model of the biological tissue; and
determining a vertical length of the fluorescent observation portion by a classic control law that is PID control law or by modern control law that is any one of optimum control law and sub-optimum control law applied to the image detected by the image detecting part,
wherein, after determining the fluorescent observation portion, the control part causes the image detecting part and the fluorescent image detecting part to detect an image of the biological tissue and a fluorescent image of a horizontal cross section of the biological tissue at multiple levels in the vertical direction within the fluorescent observation portion, said multiple levels being distributed in the vertical length of the fluorescent observation portion at a predetermined interval.
[claim2]
2. The microscope as set forth in claim 1, wherein the control part controls the stage to allow tracking of the object under observation based on at least one of the dynamic model of the object under observation and the image of the object under observation, and also controls collection of the fluorescent image of the object under observation.
[claim3]
3. The microscope as set forth in claim 1, wherein the control part controls the stage by any one of a proportional control method, an integral control method, and a derivative control method, or a classic control that is PID control by combining two or more of said control methods.
[claim4]
4. The microscope as set forth in claim 1, wherein the control part is provided with a first light source control part for controlling the first light source and a second light source control part for controlling the second light source.
[claim5]
5. The microscope as set forth in claim 1, wherein the stage is a two-dimensional or three-dimensional stage for moving the position of the object under observation.
[claim6]
6. The microscope as set forth in claim 1, wherein the fluorescent image detecting part has wavelength selecting means for isolating fluorescent light of one or more wavelengths.
[claim7]
7. The microscope as set forth in claim 1, wherein a first pinhole is provided between the second light source and the object under observation, and a second pinhole is provided between the fluorescent light and the fluorescent image detecting part.
[claim8]
8. The microscope as set forth in claim 7, wherein a pinhole drive part is provided for moving and/or rotating the first pinhole or the second pinhole.
[claim9]
9. The microscope as set forth in claim 1, wherein an objective lens is provided between the first light source and the object under observation, and an objective lens drive part is provided for driving the objective lens.
[claim10]
10. The microscope as set forth in claim 1, wherein an imaging lens is provided between a light generated at the object under observation and the image detecting part, and an imaging lens drive part is provided for driving the imaging lens.
[claim11]
11. The microscope as set forth in claim 1, wherein an imaging lens is provided between the fluorescent light and the fluorescent image detecting part, and an imaging lens drive part is provided for driving the imaging lens.
[claim12]
12. The microscope as set forth in claim 11, wherein an environmental control part is provided, and the environmental control part, which houses the object under observation, is filled with an ambient gas.
[claim13]
13. The microscope as set forth in claim 12, wherein the environmental control part has a housing part capable of housing a plurality of the objects under observation.
[claim14]
14. The microscope as set forth in claim 1, further comprising means for stimulating the object under observation.
[claim15]
15. A fluorescent observation method for a biological tissue for microscopic fluorescent observation, the method comprising: a first step of determining a fluorescent observation portion of the object under observation that is the biological tissue, the first step including: determining a center of the fluorescent observation portion along a vertical direction using at least either one of a detected image of the biological tissue formed by illumination light or a dynamic model of the biological tissue; and
determining a vertical length of the fluorescent observation portion by a classic control law that is PID control law or modern control law that is any one of optimum control law and sub-optimum control law applied to the detected image of the biological tissue formed by the illumination light, and
a second step of obtaining fluorescent images at predetermined positions within the determined fluorescent observation portion, said second step including radiating excitation light for exciting fluorescent light to the biological tissue and obtaining a fluorescent image of a horizontal cross section of the biological tissue at multiple levels in the vertical direction within the fluorescent observation portion, said multiple levels being distributed in the vertical length of the fluorescent observation portion at a predetermined interval.
[claim16]
16. The fluorescent observation method for the object under observation as set forth in claim 15, characterized in that wherein the second step includes obtaining an image of the object under observation formed by the illumination light.
[claim17]
17. The fluorescent observation method as set forth in claim 15 or 16, wherein the first and second steps are repeated after a prescribed time has passed since previous performance of the first and second steps.
[claim18]
18. The fluorescent observation method for object under observation as set forth in claim 15, wherein parameters for the dynamic model include either one of the position, speed, distribution, type, shape, ionic concentration, and molecular concentration of the object under observation, or any combinations of these.
  • 発明者/出願人(英語)
  • IGARASHI YASUNOBU
  • OBARA TAKESHI
  • DEGUCHI YUKI
  • SUZUKI TAKESHI
  • HASHIMOTO KOICHI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 348/79
参考情報 (研究プロジェクト等) SORST Selected in Fiscal 2005
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