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METHOD FOR DETERMINING CANCER ONSET OR CANCER ONSET RISK

外国特許コード F120006996
整理番号 S2010-1130-C0
掲載日 2012年10月29日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2011JP004762
国際公開番号 WO 2012/029269
国際出願日 平成23年8月26日(2011.8.26)
国際公開日 平成24年3月8日(2012.3.8)
優先権データ
  • 特願2010-196442 (2010.9.2) JP
発明の名称 (英語) METHOD FOR DETERMINING CANCER ONSET OR CANCER ONSET RISK
発明の概要(英語)

Provided is a method for highly accurately and quantitatively determining cancer onset or cancer onset risk by quantitative tissue-staining which is conducted in a biotissue with the use of an antibody capable of recognizing a cancer proliferation inhibitor or a cancer metastasis inhibitor, for example, PAR1 antibody inhibiting the mobility and infiltrating properties of cancer cells. Cancer onset or cancer onset risk is determined by a tissue staining method, said method comprising: a step for labeling an antibody, which is capable of recognizing a cancer proliferation inhibitor or a cancer metastasis inhibitor, with a fluorescent substance and then contacting the fluorescent-labeled antibody with a tissue sample

a step for irradiating a tissue site, which has been contacted with said antibody, with an excitation light to give a fluorescence image

a step for acquiring an intrinsic fluorescence image in a neighborhood region on a shorter or longer wavelength side, compared with the region wherein the wavelength of the fluorescence emitted by the fluorescent substance is acquired, in the same visual field at the same focus point as said fluorescence image

a step for conducting an image processing for removing the fluorescent brightness of said intrinsic fluorescence image from the fluorescent brightness of said fluorescence image to give a corrected fluorescence image

a step for counting cells at the tissue site which has been contacted with the antibody

a step for measuring the average fluorescent brightness per fluorescent particle

and a step for calculating the fluorescent particle count per cell.

  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOHOKU UNIVERSITY,
  • GONDA, KOHSUKE,
  • MIYASHITA, MINORU,
  • TAKEDA, MOTOHIRO,
  • OHUCHI, NORIAKI
  • 発明者(英語)
  • GONDA, KOHSUKE,
  • MIYASHITA, MINORU,
  • TAKEDA, MOTOHIRO,
  • OHUCHI, NORIAKI
国際特許分類(IPC)
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