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METHOD FOR DETERMINING EFFECTIVENESS OF MEDICINE CONTAINING ANTIBODY AS COMPONENT

外国特許コード F120006997
整理番号 S2010-1131-C0
掲載日 2012年10月29日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2011JP004763
国際公開番号 WO 2012/035705
国際出願日 平成23年8月26日(2011.8.26)
国際公開日 平成24年3月22日(2012.3.22)
優先権データ
  • 特願2010-209322 (2010.9.17) JP
発明の名称 (英語) METHOD FOR DETERMINING EFFECTIVENESS OF MEDICINE CONTAINING ANTIBODY AS COMPONENT
発明の概要(英語)

Provided is a method for determining effectiveness of medicine containing, as components, antibodies that are active components of an antibody preparation such as trastuzumab, or antibodies for a target to a target site of active component, by determining the quantity of the protein recognized by the antibodies with high accuracy, in a quantitative tissue staining method for biological tissue. The effectiveness of the medicine containing antibodies as components is determined by using the tissue staining method including the following steps: a step of labeling the antibodies in a medicine that contains antibodies as components with a fluorescent substance, and bringing the fluorescently labeled antibodies into contact with a tissue sample

a step of irradiating the tissue site contacted with the antibodies with excitation light to obtain a fluorescence image

a step of obtaining an intrinsic fluorescence image in the vicinity of the short-wavelength side or long-wavelength side of the acquisition area of fluorescence wavelength emitted from the fluorescent substance, in the same field of vision and in the same focus as those of the fluorescence image

a step of obtaining a collected fluorescence image by image-processing to eliminate the fluorescent intensity of the intrinsic fluorescence image from the fluorescent intensity of the fluorescence image

a step of counting the number of cells at the tissue site contacted with the antibodies

a step of measuring the average fluorescent intensity of one particle of fluorescent particles

and a step of calculating the number of fluorescent particles per cell.

  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOHOKU UNIVERSITY,
  • MIYASHITA, MINORU,
  • GONDA, KOHSUKE,
  • OHUCHI, NORIAKI,
  • TAKEDA, MOTOHIRO
  • 発明者(英語)
  • MIYASHITA, MINORU,
  • GONDA, KOHSUKE,
  • OHUCHI, NORIAKI,
  • TAKEDA, MOTOHIRO
国際特許分類(IPC)
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