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Nav2 channel gene-deficient mice

外国特許コード F120007010
掲載日 2012年11月19日
出願国 アメリカ合衆国
出願番号 92065301
公報番号 20020104113
公報番号 06933422
出願日 平成13年8月3日(2001.8.3)
公報発行日 平成14年8月1日(2002.8.1)
公報発行日 平成17年8月23日(2005.8.23)
優先権データ
  • 特願2000-237320 (2000.8.4) JP
  • 特願2000-241637 (2000.8.9) JP
  • 特願2001-222263 (2001.7.23) JP
発明の名称 (英語) Nav2 channel gene-deficient mice
発明の概要(英語)

An object of the present invention is to provide a null mutant non-human animal showing salt intake behavior similar to that of wild-type animals under water-sufficient conditions and showing much more intakes of hypertonic saline compared with wild-type animals under water- and salt-depleted conditions, for example, an Nav2 gene-deficient non-human animal, which is useful as a model animal of excessive salt intake experiments. The object will be attained by following process: mouse genomic libraries are screened with rat NaG cDNA as a probe, then Nav2 gene of genomic DNA is isolated, and a targeting vector is constructed by inserting marker gene such as neo gene into the exon of Nav2.

After thus constructed targeting vector is induced to ES cells, homologously recombined ES cells are selected, then germ line chimeric mice are constructed with this ES cells strain, and they are hybridized with the wild-type mice and heterozygous mutant mice are obtained. By intercrossing of thus obtained heterozygous mutant mice, Nav2 knockout mice are constructed.

  • 発明者/出願人(英語)
  • NODA MASAHARU
  • WATANABE EIJI
  • JAPAN AS REPRESENTED BY DIRECTOR OF GENERAL OF OKAZAKI NATIONAL RESEARCH INSTITUTE
  • NODA MASAHARU
  • WATANABE EIJI
国際特許分類(IPC)
米国特許分類/主・副
  • 435/69.1
  • 435/183
  • 435/320.1
  • 435/320.1
  • 435/325
  • 435/325
  • 435/455
  • 435/463
  • 536/23.2
  • 800/3
  • 800/8
  • 800/13
  • 800/18
  • 800/18
  • 800/21
  • 800/22

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