TOP > 外国特許検索 > METHOD FOR FABRICATING STABLE-ISOTOPE-LABELED TARGET PEPTIDE FRAGMENT IN MASS SPECTROMETRY

METHOD FOR FABRICATING STABLE-ISOTOPE-LABELED TARGET PEPTIDE FRAGMENT IN MASS SPECTROMETRY

外国特許コード F130007162
整理番号 S2011-0907-N0
掲載日 2013年2月14日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2012JP003965
国際公開番号 WO 2013/014853
国際出願日 平成24年6月18日(2012.6.18)
国際公開日 平成25年1月31日(2013.1.31)
優先権データ
  • 特願2011-161351 (2011.7.22) JP
発明の名称 (英語) METHOD FOR FABRICATING STABLE-ISOTOPE-LABELED TARGET PEPTIDE FRAGMENT IN MASS SPECTROMETRY
発明の概要(英語)

The present invention addresses the problem of providing a method for fabricating a stable-isotope-labeled target peptide fragment in mass spectrometry, capable of low-cost and simple fabrication. As a solution, a stable-isotope-labeled target peptide fragment in mass spectrometry is fabricated using a method comprising: a step for preparing a stable-isotope-labeled protein by causing a conjugate DNA, which is obtained between tandem-coupled DNA in which two or more DNA has been coupled in tandem for coding two or more species of target peptide fragments and DNA for coding peptide fragments for concentration measurement, to be expressed in a system in which a stable-isotope-labeled amino acid is present

a step for fragmentizing stable-isotope-labeled protein using trypsin to prepare stable-isotope-labeled peptide fragments for concentration measurement and stable-isotope-labeled target peptide fragments

a step for determining the quantity of stable-isotope-labeled peptide fragments for concentration measurement using a liquid chromatography-tandem mass spectrometry device (LC/MS/MS)

and a step for calculating the concentration of stable-isotope-labeled target peptide fragments from the quantified value of the stable-isotope-labeled peptide fragments for concentration measurement.

  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOHOKU UNIVERSITY,
  • OHTSUKI, SUMIO,
  • TERASAKI, TETSUYA
  • 発明者(英語)
  • OHTSUKI, SUMIO,
  • TERASAKI, TETSUYA
国際特許分類(IPC)
ライセンスをご希望の方、特許の内容に興味を持たれた方は、下記「問合せ先」までご連絡ください。

PAGE TOP

close
close
close
close
close
close