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HETEROLOGOUS EXPRESSION OF FUNGAL POLYKETIDE SYNTHETIC GENE IN YEAST 新技術説明会

外国特許コード F130007193
掲載日 2013年3月5日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2011JP004566
国際公開番号 WO 2012/020574
国際出願日 平成23年8月11日(2011.8.11)
国際公開日 平成24年2月16日(2012.2.16)
優先権データ
  • 特願2011-007312 (2011.1.17) JP
  • 特願2010-181279 (2010.8.13) JP
発明の名称 (英語) HETEROLOGOUS EXPRESSION OF FUNGAL POLYKETIDE SYNTHETIC GENE IN YEAST 新技術説明会
発明の概要(英語)

Provided is a method which comprises, from a gene containing an eukaryotic intron, removing the intron contained in the gene and ligating exon sequences alone to give an expression vector carrying the thus ligated sequences. Specifically provided are: a method which comprises, from a giant fungal gene containing an intron, amplifying exon sequences, as one or more fragments, by PCR, and ligating the fragments to a vector, which has been treated with a restriction enzyme, by using the gap-repair-cloning method to thereby give an expression vector containing the ligated exon sequences

a method which comprises synthesizing cDNA fragments of a giant gene and ligating the fragments to give an expression vector containing a full-length cDNA sequence

a transformant having an expression vector, which is produced by the aforesaid method, transferred therein

a protein produced by the aforesaid transformant

and a method for obtaining a compound, which is produced by the aforesaid protein, using the aforesaid expression vector.

  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • SHIZUOKA PREFECTURE PUBLIC UNIVERSITY CORPORATION,
  • NATIONAL UNIVERSITY CORPORATION OKAYAMA UNIVERSITY,
  • WATANABE, KENJI,
  • MORIYA, HISAO
  • 発明者(英語)
  • WATANABE, KENJI,
  • MORIYA, HISAO
国際特許分類(IPC)

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