METHOD FOR SCREENING SUBSTANCE RELATING TO ENDOPLASMIC RETICULUM STRESS PARTICIPATING IN ONSET OF DIABETES
The purpose of the present invention is to provide a system for evaluating the activation of an ATF6-mediated pathway. Namely, provided is a method for screening an endoplasmic reticulum stressor that is capable of activating the ATF6-mediated pathway. Also provided are a method for screening a substance that is capable of regulating endoplasmic reticulum stress induced by the activation of the ATF6-mediated pathway, and a method for screening an antidiabetic drug candidate. As the results of intensive studies, the present inventors found that the activation of the ATF6-mediated pathway can be evaluated in the state of vital cells in real time at a high sensitivity by using a polynucleotide that encodes a non-fluorescent peptide domain derived from a fluorescent protein and an ATF6 protein domain.