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MUTANT PYRROLYSYL-TRNA SYNTHETASE, AND METHOD FOR PRODUCTION OF PROTEIN HAVING NON-NATURAL AMINO ACID INTEGRATED THEREIN BY USING THE SAME

外国特許コード F130007279
整理番号 23000
掲載日 2013年4月8日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2008JP067029
国際公開番号 WO 2009/038195
国際出願日 平成20年9月19日(2008.9.19)
国際公開日 平成21年3月26日(2009.3.26)
優先権データ
  • 特願2007-243574 (2007.9.20) JP
発明の名称 (英語) MUTANT PYRROLYSYL-TRNA SYNTHETASE, AND METHOD FOR PRODUCTION OF PROTEIN HAVING NON-NATURAL AMINO ACID INTEGRATED THEREIN BY USING THE SAME
発明の概要(英語)

Disclosed is a method for introducing a lysine derivative (particularly an Ne-benzyloxycarbonyl-lysine (Z-Lys) derivative) having a useful functional group such as a heavy atom, selenium, a reactive functional group, a fluorescent group or a crosslinker, which is suitable as a non-natural amino acid, into a desired protein in a site-specific manner. Also disclosed is a mutant pyrrolysyl-tRNA shnthetase which has the substitution of at least one amino acid residue selected from a tyrosine residue at position-306, a leucine residue at position-309 and a cysteine residue at position-348 each constituting a pyrrolidin-binding site in the amino acid sequence for pyrrolysyl-tRNA shnthetase depicted in SEQ ID NO:2, wherein the substitution of the amino acid residue is the substitution of a tyrosine residue at position-306 by a glycine or alanine residue, the substitution of a leucine residue at position-309 by a glycine or alanine residue, or the substitution of a cysteine residue at position-348 by a valine, serine or alanine residue.

  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • RIKEN,
  • YOKOYAMA, SHIGEYUKI,
  • SAKAMOTO, KENSAKU,
  • YANAGISAWA, TATSUO,
  • KOBAYASHI, TAKATSUGU
  • 発明者(英語)
  • YOKOYAMA, SHIGEYUKI,
  • SAKAMOTO, KENSAKU,
  • YANAGISAWA, TATSUO,
  • KOBAYASHI, TAKATSUGU
国際特許分類(IPC)

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