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LINEAR AMPLIFICATION METHOD FOR TRACE DNA FRAGMENT

外国特許コード F130007301
整理番号 S2011-1107-N0
掲載日 2013年4月17日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2012JP074927
国際公開番号 WO 2013/047677
国際出願日 平成24年9月27日(2012.9.27)
国際公開日 平成25年4月4日(2013.4.4)
優先権データ
  • 特願2011-215318 (2011.9.29) JP
発明の名称 (英語) LINEAR AMPLIFICATION METHOD FOR TRACE DNA FRAGMENT
発明の概要(英語)

The amplification by an LM-PCR method has such a problem that amplification accuracy is not satisfactory although amplification sensitivity is high, and the amplification by a T7-IVT method has such a problem that amplification sensitivity is not satisfactory although amplification accuracy is high. The present invention provides a nucleic acid amplification method whereby it becomes possible to solve the problems of these conventional methods. A nucleic acid amplification method comprising the following step (a) and/or step (b): (a) a step of carrying out an enzymatic reaction of a terminal nucleotide transferase in the presence of DNA which cannot be added with a base at the 3'-terminal thereof with the terminal nucleotide transferase

and (b) a step of carrying out the enzymatic reaction of a Klenow enzyme in the co-presence of a poly(A-T7) oligo-DNA primer and the Klenow enzyme and in the presence of DNA that cannot undergo the formation of any double strand in a T7 region of the poly(A-T7) oligo-DNA primer.

  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY
  • 発明者(英語)
  • TANIGUCHI, HIDEKI,
  • UENO, YASUHARU
国際特許分類(IPC)
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