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TERMINATOR SEQUENCE-CONTAINING REVERSE PRIMER FOR OVEREXPRESSION AND LINEAR DNA

外国特許コード F130007355
整理番号 S2011-0594-C0
掲載日 2013年5月14日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2012JP002936
国際公開番号 WO 2012/147370
国際出願日 平成24年4月27日(2012.4.27)
国際公開日 平成24年11月1日(2012.11.1)
優先権データ
  • 特願2011-101015 (2011.4.28) JP
発明の名称 (英語) TERMINATOR SEQUENCE-CONTAINING REVERSE PRIMER FOR OVEREXPRESSION AND LINEAR DNA
発明の概要(英語)

Plasmid vectors have been widely used as a carrier of a DNA sequence capable of expressing a target RNA in cells. However, construction of these plasmid vectors requires technical skill and time. Thus, there has been required a more easy and quick method therefor. To solve this problem, a method using a linear DNA that has been amplified by the PCR method is examined. However, this method is disadvantageous in that RNA can be expressed in cells only at an extremely low level thereby. Under these circumstances, the present inventors attempted to develop an RNA expression method using a linear DNA which can be produced mainly by using the PCR method alone and which enables RNA expression at a high level. As the results of intensive studies on terminator sequences to be used in a linear DNA, the present inventors found a terminator sequence of the smallest unit which enables expression in a linear DNA equivalent to the expression with the use of a plasmid vector. A linear DNA comprising the aforesaid terminator sequence can be easily and quickly constructed and enables RNA expression at a higher level. The present invention has been completed based on these findings.

  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • YAMAGUCHI UNIVERSITY,
  • AKADA, RINJI,
  • HOSHIDA, HISASHI,
  • NAKAMURA, MIKIKO
  • 発明者(英語)
  • AKADA, RINJI,
  • HOSHIDA, HISASHI,
  • NAKAMURA, MIKIKO
国際特許分類(IPC)

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