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Alga in which production of photosynthetic products is improved, and use for said alga 新技術説明会

外国特許コード F130007432
整理番号 AF21-01WO
掲載日 2013年6月27日
出願国 アメリカ合衆国
出願番号 201113817360
公報番号 20130143260
公報番号 9051575
出願日 平成23年8月29日(2011.8.29)
公報発行日 平成25年6月6日(2013.6.6)
公報発行日 平成27年6月9日(2015.6.9)
国際出願番号 JP2011069501
国際公開番号 WO2012029727
国際出願日 平成23年8月29日(2011.8.29)
国際公開日 平成24年3月8日(2012.3.8)
優先権データ
  • 特願2010-194210 (2010.8.31) JP
  • 2011JP069501 (2011.8.29) WO
発明の名称 (英語) Alga in which production of photosynthetic products is improved, and use for said alga 新技術説明会
発明の概要(英語) Provided are an alga in which productivity of a photosynthate is increased, and use of the alga.
The alga of the present invention has an increased glutathione concentration in its chloroplast.
A method of the present invention for producing biomass is a method for producing biomass with the use of the alga of the present invention or an alga produced by a method of the present invention for producing an alga.
特許請求の範囲(英語) [claim1]
1. A method for producing an alga having an increased glutathione concentration in a chloroplast thereof, comprising the steps of: introducing, into an alga, a polynucleotide encoding a gamma -glutamylcysteine synthetase;
cultivating the modified alga into which the polynucleotide has been introduced; and
carrying out screening for the alga having the increased glutathione concentration in the chloroplast, by
(a) measuring an expression amount and/or an activity of an exogenous protein encoded by the polynucleotide and comparing the expression amount and/or the activity thus measured with an expression amount and/or an activity of an endogenous protein in a wild-type alga of a same species; or
(b) measuring the glutathione concentration in the chloroplast obtained from modified alga and comparing the glutathione concentration thus measured with the glutathione concentration in the chloroplast of a wild-type alga of the same species cultivated under same conditions.
[claim2]
2. The method according to claim 1, wherein the polynucleotide encoding the gamma -glutamylcysteine synthetase is selected from the group consisting of the following (a) to (d): (a) a polynucleotide encoding a polypeptide consisting of the amino-acid sequence set forth in SEQ ID NO: 1;
(b) a polynucleotide encoding a polypeptide which consists of an amino-acid sequence with deletion, substitution, or addition of not more than 10 amino acids in the amino-acid sequence set forth in SEQ ID NO: 1 and which has a gamma -glutamylcysteine synthetase activity;
(c) a polynucleotide consisting of the nucleotide sequence set forth in SEQ ID NO: 2; and
(d) a polynucleotide which hybridizes under a stringent condition with a polynucleotide consisting of a nucleotide sequence fully complementary to any one of the polynucleotides (a) through (c) and which encodes a polypeptide having a gamma -glutamylcysteine synthetase activity,
wherein said condition is defined by incubation overnight at 42 deg. C. in 5 * SSC and then washing in 0.1 * SSC at approximately 65 deg. C.
[claim3]
3. A method for producing biomass, comprising the step of cultivating an alga produced by the method as set forth in claim 1.
[claim4]
4. The method according to claim 3, further comprising the step of irradiating the alga with light.
[claim5]
5. The method according to claim 4, wherein the step of irradiating the alga with light is carried out under a condition where nitrogen is not substantially starved.
[claim6]
6. The method of claim 1, wherein the polynucleotide encoding the gamma -glutamylcysteine synthetase is a polynucleotide encoding a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: 1.
[claim7]
7. The method of claim 1, wherein the polynucleotide encoding the gamma -glutamylcysteine synthetase is a polynucleotide encoding a polypeptide which consists of an amino acid sequence with a deletion, a substitution, or an addition of not more than 10 amino acids in the amino acid sequence set forth in SEQ ID NO: 1 and which has a gamma -glutamylcysteine synthetase activity.
[claim8]
8. The method of claim 1, wherein the polynucleotide encoding the gamma -glutamylcysteine synthetase is a polynucleotide consisting of the nucleotide sequence set forth in SEQ ID NO: 2.
[claim9]
9. The method of claim 1, wherein the polynucleotide encoding the gamma -glutamylcysteine synthetase is a polynucleotide which hybridizes under a stringent condition defined by incubation overnight at 42 deg. C. in 5 * SSC and then washing in 0.1 * SSC at approximately 65 deg. C. with a polynucleotide consisting of a nucleotide sequence fully complementary to any one of (a) a polynucleotide consisting of the amino acid sequence set forth in SEQ ID NO: 1, (b) a polynucleotide encoding a polypeptide which consists of an amino acid sequence with deletion, substitution, or addition of one or several amino acids in the amino acid sequence set forth in SEQ ID NO: 1 and which has a gamma -glutamylcysteine synthetase activity, or (c) a polynucleotide consisting of the nucleotide sequence set forth in SEQ ID NO: 2.
[claim10]
10. The method of claim 1, wherein the alga is a sta6-positive strain of alga.
[claim11]
11. The method of claim 1, wherein the alga is a sta6-deficient strain of alga.
[claim12]
12. An alga produced by the method according to claim 1.
  • 発明者/出願人(英語)
  • OGAWA KENICHI
  • NISHIKAWA MASANOBU
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • 435/34
  • 435/101
  • 435/134
  • 435/257.2
  • 435/440
  • 435/471
参考情報 (研究プロジェクト等) CREST Creation of Innovative Technologies to Control Carbon Dioxide Emissions AREA
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