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IgA-binding peptide and purification of IgA using the same

外国特許コード F130007494
掲載日 2013年7月10日
出願国 アメリカ合衆国
出願番号 201113699579
公報番号 20130078741
公報番号 9593147
出願日 平成23年5月24日(2011.5.24)
公報発行日 平成25年3月28日(2013.3.28)
公報発行日 平成29年3月14日(2017.3.14)
国際出願番号 JP2011061906
国際公開番号 WO2011148952
国際出願日 平成23年5月24日(2011.5.24)
国際公開日 平成23年12月1日(2011.12.1)
優先権データ
  • 特願2010-118508 (2010.5.24) JP
  • 2011WO-JP61906 (2011.5.24) WO
発明の名称 (英語) IgA-binding peptide and purification of IgA using the same
発明の概要(英語) (US9593147)
This invention relates to: a peptide which comprises an amino acid sequence consisting of 12 to 18 amino acid residues represented by (X1-3)―C―(X8-10)―C―(X1-3) wherein each X independently represents an arbitrary amino acid residue other than cysteine, and C represents a cysteine residue, and is capable of binding to human IgA; and to a method for analyzing or purifying human IgA using the peptide.
特許請求の範囲(英語) [claim1]
1. A peptide which consists of any of the following amino acid sequences 1) to 7): 1) QMRCLSYKGRRVCLWL (SEQ ID NO: 2),
2) KRLCLQYKGSKVCFRL (SEQ ID NO: 4),
3) RMRCLTYRGRRVCLEL (SEQ ID NO: 5),
4) SMRCLQYRGSRVCLTL (SEQ ID NO: 6),
5) HLRCLRYKGTRVCFSL (SEQ ID NO: 8),
6) HVRCLSYKGREVCVQL (SEQ ID NO: 9), and
7) PRMCLHYKGRRVCIPY (SEQ ID NO: 10),
wherein the peptide is capable of binding to human IgA, and
wherein said peptide is optionally labeled, linked to a protein as part of a fusion protein, or bound to a solid phase.
[claim2]
2. The peptide according to claim 1, wherein the peptide has a disulfide bond between its two cysteine (C) residues.
[claim3]
3. The peptide according to claim 1, wherein the peptide is capable of binding to serum (monomeric) IgA and secretory (dimeric) IgA.
[claim4]
4. The peptide according to claim 1, wherein the peptide is labeled.
[claim5]
5. A fusion protein comprising a protein linked to a peptide according to claim 1.
[claim6]
6. An immobilized peptide comprising a peptide according to claim 1 bound to a solid phase.
[claim7]
7. A nucleic acid encoding a peptide according to claim 1.
[claim8]
8. A method for purifying IgA comprising binding IgA to A) a peptide which consists of any of the following amino acid sequences 1) to 7): 1) QMRCLSYKGRRVCLWL (SEQ ID NO: 2),
2) KRLCLQYKGSKVCFRL (SEQ ID NO: 4),
3) RMRCLTYRGRRVCLEL (SEQ ID NO: 5),
4) SMRCLQYRGSRVCLTL (SEQ ID NO: 6),
5) HLRCLRYKGTRVCFSL (SEQ ID NO: 8),
6) HVRCLSYKGREVCVQL (SEQ ID NO: 9), and
7) PRMCLHYKGRRVCIPY (SEQ ID NO: 10),
wherein the peptide is capable of binding to human IgA, or
(B) an immobilized peptide according to claim 6; and
releasing the bound IgA to collect the purified IgA.
[claim9]
9. A method for detecting IgA comprising binding IgA in a sample to A) a peptide that consists of any of the following amino acid sequences 1) to 7): 1) QMRCLSYKGRRVCLWL (SEQ ID NO: 2),
2) KRLCLQYKGSKVCFRL (SEQ ID NO: 4),
3) RMRCLTYRGRRVCLEL (SEQ ID NO: 5),
4) SMRCLQYRGSRVCLTL (SEQ ID NO: 6),
5) HLRCLRYKGTRVCFSL (SEQ ID NO: 8),
6) HVRCLSYKGREVCVQL (SEQ ID NO: 9), and
7) PRMCLHYKGRRVCIPY (SEQ ID NO: 10),
wherein the peptide is capable of binding to human IgA, or
(B) an immobilized peptide according to claim 6; and
detecting bound IgA.
[claim10]
10. A kit for analysis or purification of human IgA comprising (A) at least one peptide that consists of any of the following amino acid sequences 1) to 7): 1) QMRCLSYKGRRVCLWL (SEQ ID NO: 2),
2) KRLCLQYKGSKVCFRL (SEQ ID NO: 4),
3) RMRCLTYRGRRVCLEL (SEQ ID NO: 5),
4) SMRCLQYRGSRVCLTL (SEQ ID NO: 6),
5) HLRCLRYKGTRVCFSL (HQ ID NO: 8),
6) HVRCLSYKGREVCVQL (HQ ID NO: 9), and
7) PRMCLHYKGRRVCIPY (HQ ID NO: 10),
wherein the peptide is capable of binding to human IgA, or
(B) an immobilized peptide according to claim 6.
[claim11]
11. A column for IgA separation, comprising an immobilized peptide according to claim 6.
[claim12]
12. A peptide consisting of any of the following amino acid sequences 1) to 7): 1) QMRCLSYKGRRVCLWL (SEQ ID NO: 2),
2) KRLCLQYKGSKVCFRL (SEQ ID NO: 4),
3) RMRCLTYRGRRVCLEL (SEQ ID NO: 5),
4) SMRCLQYRGSRVCLTL (SEQ ID NO: 6),
5) HLRCLRYKGTRVCFSL (SEQ ID NO: 8),
6) HVRCLSYKGREVCVQL (SEQ ID NO: 9), and
7) PRMCLHYKGRRVCIPY (SEQ ID NO: 10),
wherein the peptide is capable of binding to human IgA.
  • 発明者/出願人(英語)
  • ITO YUJI
  • KAGOSHIMA UNIVERSITY
  • OTSUKA CHEMICAL
国際特許分類(IPC)
米国特許分類/主・副
  • 436/501
  • 530/326
  • 530/350
  • 530/387.1
  • 536/23.1
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