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METHOD FOR PREPARING AMNIOTIC MESENCHYMAL STEM CELL, AND ISOLATED AMNIOTIC MESENCHYMAL STEM CELL MASS

外国特許コード F130007708
掲載日 2013年11月27日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2012JP080375
国際公開番号 WO 2013/077428
国際出願日 平成24年11月22日(2012.11.22)
国際公開日 平成25年5月30日(2013.5.30)
優先権データ
  • 特願2011-257419 (2011.11.25) JP
発明の名称 (英語) METHOD FOR PREPARING AMNIOTIC MESENCHYMAL STEM CELL, AND ISOLATED AMNIOTIC MESENCHYMAL STEM CELL MASS
発明の概要(英語)

An amniotic mesenchymal stem cell mass having a high proliferation ability and a high differentiation ability can be produced by a method comprising the steps of: (A) collecting a cell mass of a mesenchymal cell from the amnion of a mammal

(B) subjecting the cell mass of the mesenchymal cell to flow cytometry to thereby sort cells, wherein a specific gate is provided in a scattergram produced in the flow cytometry

(C) and subjecting the sorted cells to subculture. Alternatively, the amniotic mesenchymal stem cell mass having a high proliferation ability and a high differentiation ability can also be produced by a method comprising the steps of: (D) collecting a cell mass of a mesenchymal cell from the amnion of a mammal

(E) subjecting the collected cell mass to initial culture for 2 to 3 days

(F) repeating the subculture of the resultant cell mass three to four times at a low cell concentration

and (G) maintaining the culture of the cells in the same culture dish until the cells become confluent when a fusiform colony of the cells is formed in the subculture.

  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • UNIVERSITY OF TOYAMA
  • 発明者(英語)
  • NIKAIDO, TOSHIO,
  • YOSHIDA, TOSHIKO,
  • OKABE, MOTONORI,
  • KOIKE, CHIKA,
  • NOGAMI, MAKIKO,
  • KIMURA, TOMOATSU,
  • NOGUCHI, MAKOTO,
  • TSUNO, HIROAKI,
  • TAKEDA, YUJI
国際特許分類(IPC)
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