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Planar patch clamp device, electrodes for said device and cell ion channel current measurement method 新技術説明会

外国特許コード F140007816
整理番号 AF19-03TW
掲載日 2014年1月20日
出願国 台湾
出願番号 101148766
公報番号 201339300
公報番号 I558811
出願日 平成24年12月20日(2012.12.20)
公報発行日 平成25年10月1日(2013.10.1)
公報発行日 平成28年11月21日(2016.11.21)
優先権データ
  • 特願2011-278445 (2011.12.20) JP
  • 特願2012-181786 (2012.8.20) JP
発明の名称 (英語) Planar patch clamp device, electrodes for said device and cell ion channel current measurement method 新技術説明会
発明の概要(英語) (TWI558811)
In order to reduce a noise current by suppressing variations in an applied membrane potential in a planar patch clamp device, enabling an ion channel current to be accurately measured, this planar patch clamp device is provided with a liquid reservoir that has at least one fine through-hole disposed on an electrically insulative substrate, and holds a conductive liquid on surfaces on both sides of the through-hole; and electrodes, which are capable of being energized, are disposed in said liquid reservoir.
The electrodes are provided with: (a) an electrode vessel, wherein part of the vessel wall comprises an inorganic porous material; (b) an electrode, wherein a noble metal chloride (NmCl) layer is formed on the surface of the noble metal (Nm); and (c) an electrode solution, wherein the noble metal chloride and an alkali metal chloride are dissolved at a saturated concentration.
特許請求の範囲(英語) [claim1]
1. Kinds of plane diaphragm pliers installments, its characteristic is: (1) the electric insulation foundation plate establishment has over 1 causes its both sides superficial connections, may cause the cell not through, but causes the liquid through the tiny birthplace through hole, the (2) above birthplace through hole's first superficial side establishes to be useful with the second superficial side separately in maintaining the conductive liquid storing fluid department and with may to store fluid department the electricity pole piece of conductive liquid circular telegram disposition, (3) first superficial side's storing fluid department as the storing fluid department that the cell disposition uses, then the (4) above first superficial side and second superficial side's electricity pole piece has the following (a)-(c) essential factor: when (a) above stores in the fluid department inducts the conductive liquid with this conductive liquid contact at least one part of vessel wall by the electrode vessel of inorganic porous material constitution, the (b) info clerk in the above electrode vessel, the precious metal Nm surface layer department forms this precious metal the chloride NmCl level electrode, (c) fills in the above electrode vessel, by the saturated density dissolution has above precious metal chloride NmCl and alkaline metal chloride electrode solution.
[claim2]
2. According to claim 1, plane diaphragm pliers installment, above first superficial side storing fluid department including material constitution by light transmission for disposing the cell's 1st liquid storage tank and disposition has first superficial side the 2nd liquid storage tank and with making the above host liquid storage tank and narrow liquid circuit of vice-liquid storage tank of connection electricity pole piece.
[claim3]
3. According to claim 1, or 2 plane diaphragm pliers installment, above second superficial side storing fluid department with for inducting and discharging the liquid circuit connection of conductive liquid, this liquid circuit disposition has the second superficial side electricity pole piece.
[claim4]
4. Like the application patent scope 1-3rd any plane diaphragm pliers installment, the above first superficial side establishment has many to store the fluid department, these store in the fluid department to form the concave department, this concave department has compared to nerve cell's cell body great width with may prevent to raise the condition cell body the depth of migration.
[claim5]
5. Like the application patent scope 1-4th any plane diaphragm pliers installment, the above plane diaphragm pliers installment has the constitution of following (A) and/or (B): the (A) second surface side's storing fluid department and liquid suction unit connection, may through this liquid suction unit's storing fluid department load negative pressure to the second superficial side, (B) in foundation plate's birthplace through hole the opening oral area in first superficial side peripheral, adheres to stick cohere has to have the cell fixed strength extracellular matrix to form the material.
[claim6]
6. 1 kinds of plane diaphragm pliers installment electricity pole pieces, its characteristic to have below (a') - (c') essential factor: (A') at least one part of vessel wall by the electrode vessel of inorganic porous material constitution, (b') info clerk's precious metal Nm surface layer department above electrode vessel forms this precious metal the chloride NmCl level electrode, (c') packing in the above electrode vessel has above precious metal chloride NmCl and alkaline metal chloride electrode solution by the saturated density dissolution.
[claim7]
7. According to claim 6, item of plane diaphragm pliers installment electricity pole piece, constitution above vessel wall's at least one part of inorganic porous material for sintered glass or porous ceramic.
[claim8]
8. According to claim 6, or 7 items of plane diaphragm pliers installment electricity pole pieces, above precious metal Nm is silver Ag or platinum Pt, alkaline metal chloride for potassium chloride KCl.
[claim9]
9. Like the application patent scope 6-8th item of any item of above plane diaphragm pliers installment electricity pole piece, above electrode for following (C) or (D):(C) prominent clavate electrode in electrode vessel, is formed the precious metal chloride NmCl level by precious metal Nm constitution the surface layer department of core material's, (D) in the tube shape electrode that electrode vessel's wall department's in Zhou Mian form, vessel Bi Buce first floor for the precious metal Nm evaporation level, with surface layer of electrode solution contact for the precious metal chloride NmCl evaporation level.
[claim10]
10. 1 cell ion channel current measurement methods, its characteristic lies, to application patent scope 1-5th any above plane diaphragm pliers installment the storing fluid department of second superficial side's inducts the conductive liquid at the same time, inducts the dispersion to have the achievement to determine the object to the first superficial side's storing fluid department cell's conductive liquid, and causes the first superficial side storing fluid department with the storing fluid department conductively connection of second superficial side, the above cell disposition in first superficial side storing fluid department's position, based on this and second superficial side electricity pole piece to the first superficial sideAmong the electrodes exerts the voltage, surveys as the determination thing cell's ion channel electric current.
[claim11]
11. According to claim 10, cell ion channel current measurement method, above plane diaphragm pliers installment the first superficial side establishment of foundation plate has the application patent scope 4th item of institute to state the constitution many to store the fluid department, as the determination object's cell is the nerve cell, the conductive liquid is the above nerve cell's cell nutrient fluid.
[claim12]
12. According to claim 11, cell ion channel current measurement method, in the above nerve cell inducts in advance is useful in the Ca image formation Ca probe, at least when through including the transmission of occurrence when or activity electric potential of observation cell activity electric potential the fluorescence Ca image formation's way that has determines this cell ion channel electric current.
[claim13]
13. According to claim 12, cell ion channel current measurement method, will induct has the above Ca probe's nerve cell many or the massive dispositions in the above plane diaphragm pliers installment first superficial side at the same time, forms nerve cell network that the synapse interconnections of these nerve cells become, the sole nerve cell injection current or exerts the voltage to them, from this carries on the determination through above many or the massive nerve cell's above Ca image formation.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • URISU TSUNEO
  • WANG ZHI-HONG
  • UNO HIDETAKA
  • OBULIRAJ SENTHIL KUMAR
  • NAGAOKA YASUTAKA
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Creation of Nanosystems with Novel Functions through Process Integration AREA
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